CHOLESTEROL ESTERASE
Synonym(s):CE, Sterolester Acylhydrolase;Cholesterol Esterase from Pseudomonas fluorescens;Cholesterol Esterase from hog pancreas;Cholesterol Esterase, Microbial;Sterol-ester acylhydrolase
- CAS NO.:9026-00-0
- Empirical Formula: NULL
- Molecular Weight: 0
- MDL number: MFCD00071052
- EINECS: 232-808-6
- Update Date: 2023-05-04 15:15:42
What is CHOLESTEROL ESTERASE?
Chemical properties
Powder
The Uses of CHOLESTEROL ESTERASE
Cholesterol Esterase is used for enzymatic determination of total cholesterol when coupled with cholesterol oxidize in clinical analysis. Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols.
The Uses of CHOLESTEROL ESTERASE
Cholesterol Esterase from porcine pancreas has been used:
- in an in vitro simulated digestion model to improve the hydrolysis of carotenoid esters in the intestinal phase
- to analyze the bioavailability of phytosterol and cholesterol in the aqueous micellar phase of an in vitro simulated digestion model
- as a standard in enzyme activity assay
The Uses of CHOLESTEROL ESTERASE
Cholesterol Esterase from bovine pancreas has been used:
- in γ-oryzanol-hydrolyzing activity
- in cholesterol esterase enzyme activity for quantification of cholesterol and cholesterol esters associated in tear film and lenses samples
- to test in vitro degradation of poly(fumaroyl bioxirane) maleate (PFM) polymer designed for bone tissue engineering
General Description
Cholesterol Esterase is a serine hydrolase released from the exocrine pancreas. It belongs to the α/β hydrolase family of enzymes.
Biochem/physiol Actions
Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramides. The enzyme may have multiple functions in lipid and lipoprotein metabolism, as well as in atherosclerosis. Its molecular mass is approximately 129 kDa and the optimum pH range is 7.0-9.0. The enzyme is activated by cholic acid, glycocholic acid, BSA, Mg2+ and 0.3% (v/v) ? X-100. Ag+, Hg2+ and ionic detergents inhibit the activity of the enzyme.
Properties of CHOLESTEROL ESTERASE
vapor pressure | 0.004Pa at 25℃ |
storage temp. | -20°C |
solubility | Dissolve 1mg/ml in 0.1M phosphate buffer, pH 6.0 to 7.0 |
form | powder |
color | white |
EPA Substance Registry System | Esterase, cholesterol (9026-00-0) |
Safety information for CHOLESTEROL ESTERASE
Computed Descriptors for CHOLESTEROL ESTERASE
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