Alizarin Red S

Chemical properties

yellow-orange powder

The Uses of Alizarin Red S

Alizarin Red S is used in the biochemical assay to determine the presence of calcium.

The Uses of Alizarin Red S

Alizarin Red S sodium salt is used as a chromogenic agent for the colorimetric determination of dothiepin hydrochloride in pharmaceutical formulations. It is also used as an ion-pair reagent for the spectrophotometric assay of fexofenadine in pharmaceuticals. It acts as a colorometric pH indicator in the range of 3.8-5.4. Further, it is used in a biochemical assay to determine the presence of calcific deposition by cells of an osteogenic lineage.

What are the applications of Application

Alizarin Red S is used to determine the presence of calcific deposition by cells of an osteogenic lineage

Definition

ChEBI: An organic sodium salt having 3,4-dihydroxy-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate as the counterion. It is commonly used to stain embryo skeletons in cleared whole mounts, usually of small mammals.

Preparation

Alizarin Red S was prepared by reacting 1,2-Dihydroxyanthracene-9,10-dione with fuming sulfuric acid sulphonate, and translated into Sodium salt.

Biochem/physiol Actions

Alizarin Red S is used for the staining of cartilage and bone. It reacts with calcium, thereby helping in the diagnosis of calcium deposits. It is also used to study carbohydrate–boronic acid interactions.

Properties and Applications

dark blue light red (chromium), brilliant yellow red (aluminum). Claybank powder. Soluble in Cellosolve, slightly soluble in ethanol and Acetone. In concentrated sulfuric acid to orange, dilution to pale yellow.