Alizarin Red S

Synonym(s):3,4-Dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt;Alizarin Carmine;Alizarin Red, water soluble;Alizarin sodium monosulfonate;Alizarin sulfonate sodium

CAS NO.：130-22-3
Empirical Formula: C14H7NaO7S
Molecular Weight: 342.26
MDL number: MFCD00013049
EINECS: 204-981-8
SAFETY DATA SHEET (SDS)
Update Date: 2025-12-17 17:32:32

What is Alizarin Red S?

Chemical properties

yellow-orange powder

The Uses of Alizarin Red S

Alizarin Red S is used in the biochemical assay to determine the presence of calcium.

The Uses of Alizarin Red S

Alizarin Red S sodium salt is used as a chromogenic agent for the colorimetric determination of dothiepin hydrochloride in pharmaceutical formulations. It is also used as an ion-pair reagent for the spectrophotometric assay of fexofenadine in pharmaceuticals. It acts as a colorometric pH indicator in the range of 3.8-5.4. Further, it is used in a biochemical assay to determine the presence of calcific deposition by cells of an osteogenic lineage.

What are the applications of Application

Alizarin Red S is used to determine the presence of calcific deposition by cells of an osteogenic lineage

Definition

ChEBI: An organic sodium salt having 3,4-dihydroxy-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate as the counterion. It is commonly used to stain embryo skeletons in cleared whole mounts, usually of small mammals.

Preparation

Alizarin Red S was prepared by reacting 1,2-Dihydroxyanthracene-9,10-dione with fuming sulfuric acid sulphonate, and translated into Sodium salt.

Biochem/physiol Actions

Alizarin Red S is used for the staining of cartilage and bone. It reacts with calcium, thereby helping in the diagnosis of calcium deposits. It is also used to study carbohydrate–boronic acid interactions.

Properties and Applications

dark blue light red (chromium), brilliant yellow red (aluminum). Claybank powder. Soluble in Cellosolve, slightly soluble in ethanol and Acetone. In concentrated sulfuric acid to orange, dilution to pale yellow.

Standard	Ironing Fastness	Light Fastness	Fulling		Persperation Fastness	Soaping	Water
Standard	Ironing Fastness	Light Fastness	Alkali	Acid	Persperation Fastness	Soaping	Water
ISO	3	6	4-5	1	4-5	5	5
AATCC	3-4	6-7	3		5	4	4

Properties of Alizarin Red S

Melting point:	>250°C
Boiling point:	287-289°C
storage temp.	room temp
solubility	H2O: soluble1mg/mL
form	Powder
pka	4.5, 11(at 25℃)
Colour Index	58005
color	Various
PH Range	4.3(yellow)-6.3(violet)
Water Solubility	Soluble in water and alcohol.
λmax	556nm, 596nm, 423nm, 546nm
Merck	14,8573
BRN	4117090
Stability:	Stable. Incompatible with strong oxidizing agents.
Biological Applications	Detecting Candida,lactic acid bacteria,microorganisms; treating gastropathy,viral diseases
Major Application	Nanocomposite material, chemical mechanical polishing, optical fibers, display device, carbon nanotubes, textiles, determination of aluminum,iron,copper,manganese,zinc,calcium,magnesium,zirconium,hafnium,molybdenum,uranium,vanadium, detergents, hair dyes, determination of proteins,clotrimazole,ketoconazole,piroxicam,and tenoxicam
CAS DataBase Reference	130-22-3(CAS DataBase Reference)
EPA Substance Registry System	Sodium alizarinesulfonate (130-22-3)

Safety information for Alizarin Red S

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.