ACYL-COENZYME A SYNTHETASE
Synonym(s):Acid: CoA ligase (AMP-forming)
- CAS NO.:9013-18-7
- Molecular Weight: 0
- MDL number: MFCD00130343
- EINECS: 232-747-5
- Update Date: 2023-08-18 14:52:35
What is ACYL-COENZYME A SYNTHETASE?
Chemical properties
White powder
The Uses of ACYL-COENZYME A SYNTHETASE
Acyl-coenzyme A (coA) synthetase from Pseudomonas sp. has been used:
- as ligase in the synthesis of mevalonate derivatives of adenosine triphosphate (ATP)
- in in vitro fatty acylation assays
- in the synthesis of (14C)Vernoloyl-CoA (Va-CoA),(3) bisphosphonates derivatives of ATP(4) and (3H)Palmitate-CoA
General Description
Acyl-coenzyme A synthetase belongs to adenylate-forming enzymes superfamily. It has a conserved adenosine triphosphate/adenosine monophosphate (ATP/AMP) binding motif.
Biochem/physiol Actions
Acyl coenzyme A synthetase proteins are involved in regulating and facilitating long-chain fatty acid transport in mammalian cells .
Purification Methods
Purify the synthase by extraction with sucrose/HCO3 buffer, protamine sulfate precipitation, (NH4)2SO4 (66-65%) fractination (pH 4.35) and a second (NH4)2SO4 (35-60%) fractionation (pH 4.35). It has Km 0.15mM (Vrel 1.0) for octanoate and 0.41mM (Vrel 2.37) for heptanoate. The Km for ATP is 0.5mM, all at pH 9.0 in ethylene glycol buffer at 38o. [Jencks et al. J Biol Chem 204 453 1953, Methods Enzymol 5 467 1962.]
Properties of ACYL-COENZYME A SYNTHETASE
storage temp. | -20°C |
form | powder |
color | white |
Safety information for ACYL-COENZYME A SYNTHETASE
Computed Descriptors for ACYL-COENZYME A SYNTHETASE
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