Fluorescein
Synonym(s):Acid Yellow 73
- CAS NO.:2321-07-5
- Empirical Formula: C20H12O5
- Molecular Weight: 332.31
- MDL number: MFCD00005050
- EINECS: 219-031-8
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-05-13 18:00:15
What is Fluorescein?
Absorption
Rapidly distributed
Description
Fluorescein is a synthetic organic compound available as a dark orange/red powder slightly soluble in water and alcohol. It is widely used as a fluorescent tracer for many applications.
Fluorescein is a fluorophore commonly used in microscopy, in a type of dye laser as the gain medium, in forensics and serology to detect latent blood stains, and in dye tracing. Fluorescein has an absorption maximum at 494 nm and emission maximum of 521 nm (in water). The major derivatives are fluorescein isothiocyanate (FITC) and, in oligonucleotide synthesis, 6-FAM phosphoramidite.
Fluorescein also has an isosbestic point (equal absorption for all pH values) at 460 nm. Fluorescein is also known as a color additive (D&C Yellow no. 7). The disodium salt form of fluorescein is known as uranine or D&C Yellow no. 8.
The color of its aqueous solution varies from green to orange as a function of the way it is observed: by reflection or by transmission, as it can be noticed in bubble levels in which fluorescein is added as a colorant to the alcohol filling the tube to increase the visibility of the air bubble and the precision of the instrument. More concentrated solutions of fluorescein can even appear red.
Description
Fluorescein is an organic dye also known as D & C Yellow No. 7 and many other names. A. von Baeyer first synthesized it in 1871 from phthalic anhydride and resorcinol and called it resorcinphthalein. It is not soluble in water or many organic solvents, but its disodium salt (fluorescein sodium, uranine, D & C Yellow No. 8) is highly water-soluble.
Crystalline or powdered fluorescein is reddish orange, but in alkaline solution it has an intense yellow-green fluorescence. Because of its fluorescence, it was used to dye the Chicago River for St. Patrick’s Day, beginning in 1962. A few years later, it was replaced by a more environmentally friendly vegetable dye.
The Uses of Fluorescein
Fluorescein is used as a fluorescent tracer for many applications including in a type of dye laser as the gain medium, in forensics and serology to detect latent blood stains and in dye tracing. It is used to localise multiple muscular ventricular septal defects during open heart surgery and confirm the presence of any residual defects. It is applied to teeth to reveal plaque.
Background
A phthalic indicator dye that appears yellow-green in normal tear film and bright green in a more alkaline medium, such as the aqueous humor, and is used therapeutically as a diagnostic aid in corneal injuries and corneal trauma. It has been approved by FDA for use in externally applied drugs and cosmetics. (From Merck Index, 12th ed; American Medical Association Drug Evaluations; 1995, p2275)
Indications
For diagnostic imaging. Primarily indicated in diagnostic fluorescein angiography or angioscopy of the fundus and of the iris vasculature.
What are the applications of Application
Fluorescein (free acid) is the free acid form of fluorescein
Metabolism
Not Available
Properties of Fluorescein
Melting point: | 320 °C(lit.) |
Boiling point: | 429.44°C (rough estimate) |
Density | 1.2739 (rough estimate) |
storage temp. | room temp |
solubility | Solubility Insoluble in water, ether, benzene, chloroform; soluble in ethanol, methanol, acetone, Iethyl acetate, N,N-dimethylformamide |
form | Powder |
color | Red to orange |
Water Solubility | insoluble |
Safety information for Fluorescein
Signal word | Warning |
Pictogram(s) |
Exclamation Mark Irritant GHS07 |
GHS Hazard Statements |
H319:Serious eye damage/eye irritation |
Precautionary Statement Codes |
P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. |
Computed Descriptors for Fluorescein
Abamectin manufacturer
Arnish Laborates Pvt Ltd
Rivashaa Agrotech Biopharma Pvt. Ltd.
GSP Dyes and Chemicals Pvt.Ltd
Garuda Chemicals
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