5-Bromo-4-chloro-3-indolyl-beta-D-galactoside
Synonym(s):X-Gal;BCIG;bromo-4-chloro-3-indolyl-β-d-galactopyranoside, 5-;5-Bromo-4-chloro-3-indolyl β-D -galactopyranoside;5-Bromo-4-chloro-3-indolyl β-D -galactoside
- CAS NO.：7240-90-6
- Empirical Formula: C14H15BrClNO6
- Molecular Weight: 408.63
- MDL number: MFCD00005666
- EINECS: 230-640-8
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-07-01 14:39:02
What is 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside?
Chemical properties
White Powder
The Uses of 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside
A substrate for β-Galactosidase that is converted by the enzyme into an intense indigo-blue chromophore (615 nm).
The Uses of 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside
magenta b-galactosidase substrate
The Uses of 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside
A substrate for -Galactosidase that is converted by the enzyme into an intense indigo-blue chromophore (615 nm)
The Uses of 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside
In histochemical staining, in blue-white selection of bacterial colonies with lacZ gene activity during cloning experiments.
What are the applications of Application
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside is a chromogenic histochemical substrate for β-galactosidase
Definition
ChEBI: An indolyl carbohydrate that is the beta-D-galactoside of 3-hydroxy-1H-indole in which the indole moiety is substituted at positions 4 and 5 by chlorine and bromine, respectively. It is used to test for he presence of an enzyme, beta-galactosidase, which cleaved the glycosidic bond to give 5-bromo-4-chloro-3-hydroxy-1H-indole, which immediately dimerises to give an intensely blue product.
storage
+4°C
Properties of 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside
| Melting point: | 230 °C |
| Boiling point: | 673.9±55.0 °C(Predicted) |
| alpha | -64 º (C=1, H2O/DMF (1/1)) |
| Density | 1.5563 (rough estimate) |
| refractive index | 1.6110 (estimate) |
| storage temp. | -20°C |
| solubility | Soluble in 100 mM in DMSO. |
| form | Powder |
| pka | 12.74±0.70(Predicted) |
| color | White |
| Odor | Odorless |
| Sensitive | Moisture & Light Sensitive |
| Merck | 14,10074 |
| BRN | 1402009 |
| Stability: | Stable. Incompatible with strong oxidizing agents. |
| CAS DataBase Reference | 7240-90-6(CAS DataBase Reference) |
Safety information for 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside
| Signal word | Warning |
| Pictogram(s) |
 Exclamation Mark Irritant GHS07 |
| Precautionary Statement Codes |
P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P270:Do not eat, drink or smoke when using this product. P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection. P312:Call a POISON CENTER or doctor/physician if you feel unwell. P330:Rinse mouth. P363:Wash contaminated clothing before reuse. P302+P352:IF ON SKIN: wash with plenty of soap and water. P304+P340:IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. P403:Store in a well-ventilated place. |
Computed Descriptors for 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside
| InChIKey | OPIFSICVWOWJMJ-AEOCFKNESA-N |
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