4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE

Synonym(s):4-Nitrophenyl phosphate disodium salt hexahydrate;di-Sodium 4-nitrophenyl phosphate;Disodium 4-nitrophenyl phosphate hexahydrate;Phosphatase substrate;pNPP disodium hexahydrate

CAS NO.：333338-18-4
Empirical Formula: C6H9NNaO7P
Molecular Weight: 261.1
MDL number: MFCD00066288
EINECS: 224-246-5
SAFETY DATA SHEET (SDS)
Update Date: 2024-10-28 23:16:16

4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE Structural

What is 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE?

Chemical properties

White to off-white powder

The Uses of 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE

Substrate for alkaline and acid phosphatase determinations4-Nitrophenyl phosphate disodium salt hexahydrate is a chromogenic substrate used in the determination of acid and alkaline phosphatases.

The Uses of 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE

4-Nitrophenyl phosphate disodium salt hexahydrate has been used in:

enzyme-linked immunosorbent assay (ELISA) to visualize glycoprotein VI (GPVI) binding to collagen type I (Col I)
enzymatic color reaction for semi-quantitative measurement of allergen-specific IgE by ELISA
alkaline phosphatase assay

The Uses of 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE

Phosphatase substrate has been used in the chromogenic detection of adsorbed rabbit IgG to determine coating efficiency.

General Description

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Biochem/physiol Actions

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol. pNPP is a common chromogenic substrate for alkaline phosphatase.

Properties of 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE

Melting point:	>300 °C(lit.)
storage temp.	-20°C
solubility	H₂O: 25 mg/mL at 20 °C, clear, yellow-green
form	tablets
color	white to pale yellow
PH	pH (50g/l, 25℃) : 8.0～9.0
Water Solubility	almost transparency
BRN	5324661
CAS DataBase Reference	333338-18-4(CAS DataBase Reference)

Safety information for 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection.