4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
Synonym(s):4-Nitrophenyl phosphate disodium salt hexahydrate;di-Sodium 4-nitrophenyl phosphate;Disodium 4-nitrophenyl phosphate hexahydrate;Phosphatase substrate;pNPP disodium hexahydrate
- CAS NO.：333338-18-4
- Empirical Formula: C6H9NNaO7P
- Molecular Weight: 261.1
- MDL number: MFCD00066288
- EINECS: 224-246-5
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-10-28 23:16:16
What is 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE?
Chemical properties
White to off-white powder
The Uses of 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
Substrate for alkaline and acid phosphatase determinations4-Nitrophenyl phosphate disodium salt hexahydrate is a chromogenic substrate used in the determination of acid and alkaline phosphatases.
The Uses of 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
4-Nitrophenyl phosphate disodium salt hexahydrate has been used in:
- enzyme-linked immunosorbent assay (ELISA) to visualize glycoprotein VI (GPVI) binding to collagen type I (Col I)
- enzymatic color reaction for semi-quantitative measurement of allergen-specific IgE by ELISA
- alkaline phosphatase assay
The Uses of 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
Phosphatase substrate has been used in the chromogenic detection of adsorbed rabbit IgG to determine coating efficiency.
General Description
Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.
Biochem/physiol Actions
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol. pNPP is a common chromogenic substrate for alkaline phosphatase.
Properties of 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
| Melting point: | >300 °C(lit.) |
| storage temp. | -20°C |
| solubility | H2O: 25 mg/mL at 20 °C, clear, yellow-green |
| form | tablets |
| color | white to pale yellow |
| PH | pH (50g/l, 25℃) : 8.0～9.0 |
| Water Solubility | almost transparency |
| BRN | 5324661 |
| CAS DataBase Reference | 333338-18-4(CAS DataBase Reference) |
Safety information for 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
| Signal word | Warning |
| Pictogram(s) |
 Exclamation Mark Irritant GHS07 |
| GHS Hazard Statements |
H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation |
| Precautionary Statement Codes |
P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection. |
Computed Descriptors for 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
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