4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
Synonym(s):4-Nitrophenyl phosphate disodium salt hexahydrate;di-Sodium 4-nitrophenyl phosphate;Disodium 4-nitrophenyl phosphate hexahydrate;Phosphatase substrate;pNPP disodium hexahydrate
- CAS NO.：333338-18-4
- Empirical Formula: C6H9NNaO7P
- Molecular Weight: 261.1
- MDL number: MFCD00066288
- EINECS: 224-246-5
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-10-28 23:16:16
What is 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE?
Chemical properties
White to off-white powder
The Uses of 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
Substrate for alkaline and acid phosphatase determinations4-Nitrophenyl phosphate disodium salt hexahydrate is a chromogenic substrate used in the determination of acid and alkaline phosphatases.
The Uses of 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
4-Nitrophenyl phosphate disodium salt hexahydrate has been used in:
- enzyme-linked immunosorbent assay (ELISA) to visualize glycoprotein VI (GPVI) binding to collagen type I (Col I)
- enzymatic color reaction for semi-quantitative measurement of allergen-specific IgE by ELISA
- alkaline phosphatase assay
The Uses of 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
Phosphatase substrate has been used in the chromogenic detection of adsorbed rabbit IgG to determine coating efficiency.
General Description
Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.
Biochem/physiol Actions
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol. pNPP is a common chromogenic substrate for alkaline phosphatase.
Properties of 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
| Melting point: | >300 °C(lit.) |
| storage temp. | -20°C |
| solubility | H2O: 25 mg/mL at 20 °C, clear, yellow-green |
| form | tablets |
| color | white to pale yellow |
| PH | pH (50g/l, 25℃) : 8.0～9.0 |
| Water Solubility | almost transparency |
| BRN | 5324661 |
| CAS DataBase Reference | 333338-18-4(CAS DataBase Reference) |
Safety information for 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
| Signal word | Warning |
| Pictogram(s) |
 Exclamation Mark Irritant GHS07 |
| GHS Hazard Statements |
H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation |
| Precautionary Statement Codes |
P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection. |
Computed Descriptors for 4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
New Products
4-Aminotetrahydropyran-4-carbonitrile Hydrochloride (R)-3-Aminobutanenitrile Hydrochloride 4-AMINO-TETRAHYDRO-PYRAN-4-CARBOXYLIC ACID HCL 4-(Dimethylamino)tetrahydro-2H-pyran-4-carbonitrile 3-((Dimethylamino)methyl)-5-methylhexan-2-one oxalate 1,4-Dioxa-8-azaspiro[4.5]decane 5-Bromo-2-nitropyridine Nimesulide BP Aceclofenac IP/BP/EP Diclofenac Sodium IP/BP/EP/USP Mefenamic Acid IP/BP/EP/USP Ornidazole IP Diclofenac Potassium SODIUM AAS SOLUTION ZINC AAS SOLUTION BUFFER SOLUTION PH 10.0(BORATE) GOOCH CRUCIBLE SINTERED AQUANIL 5 BERYLLIUM AAS SOLUTION 2-Bromo-1-(bromomethyl)-3-chloro-5-nitrobenzene 2-Bromo-3-nitroaniline N-(3-Hydroxypropyl)-N-methylacetamide 3-Bromo-6-chloropyridazine 4-ethyl-3-nitrobenzoic acidRelated products of tetrahydrofuran
You may like
-
Disodium 4-Nitrophenyl Phosphate Hexahydrate CAS 333338-18-4View Details
333338-18-4 -
4-NITROPHENYLPHOSPHATE DISODIUM SALT CAS 333338-18-4View Details
333338-18-4 -
4-Nitrophenyl phosphate disodium salt hexahydrate CAS 333338-18-4View Details
333338-18-4 -
Phosphatase substrate CAS 333338-18-4View Details
333338-18-4 -
Phosphatase substrate CAS 333338-18-4View Details
333338-18-4 -
Phosphatase substrate CAS 333338-18-4View Details
333338-18-4 -
4-Nitrophenyl phosphate disodium salt hexahydrate CAS 333338-18-4View Details
333338-18-4 -
Phosphatase substrate CAS 333338-18-4View Details
333338-18-4
Statement: All products displayed on this website are only used for non medical purposes such as industrial applications or scientific research, and cannot be used for clinical diagnosis or treatment of humans or animals. They are not medicinal or edible.