4-(2-Aminoethyl)benzenesulfonylfluoride hydrochloride

Synonym(s):AEBSF;4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride;4-(2-Aminoethyl)benzenesulfonylfluoride, HCl, 4-(2-Aminoethyl)benzene-1-sulfonyl fluoride, HCl;4-(2-Aminoethyl)benzenesulfonylfluoride, HCl, 4-(2-Aminoethyl)benzene-1-sulfonylfluoride, HCl, AEBSF, HCl InSolution;(4-(2-aminoetyl)-benzenesulfonyl fluoride hydrochloride)

CAS NO.：30827-99-7
Empirical Formula: C8H11ClFNO2S
Molecular Weight: 239.69
MDL number: MFCD00132962
EINECS: 608-547-2
SAFETY DATA SHEET (SDS)
Update Date: 2023-10-09 22:51:44

4-(2-Aminoethyl)benzenesulfonylfluoride hydrochloride Structural

What is 4-(2-Aminoethyl)benzenesulfonylfluoride hydrochloride?

Description

AEBSF is a water soluble, irreversible, broad spectrum inhibitor of serine proteases, including trypsin, chymotrypsin, plasmin, thrombin, and kallikreins. AEBSF can also prevent the activation of the ROS generator, NADPH oxidase. At 10-50 μg, AEBSF can attenuate airway inflammation in a mouse model of airway allergy. AEBSF maintains stability in slightly acidic aqueous solutions and serves as a nontoxic alternative to the organophosphate inhibitors, PMSF and DFP.

Chemical properties

white powder

The Uses of 4-(2-Aminoethyl)benzenesulfonylfluoride hydrochloride

AEBSF is extensively used in studies aiming to describe cholesterol regulatory genes due to its potent ability to inhibit Site-1-protease (S1P). This serine protease, located in the Golgi apparatus, is responsible for activating the sterol regulatory element-binding proteins. By selectively inhibiting S1P, AEBSF can be used to characterize the downstream result of SREBP inhibition and its influence on cholesterol regulation.

The Uses of 4-(2-Aminoethyl)benzenesulfonylfluoride hydrochloride

Recommended concentrations for use are 0.1-1 mM.

What are the applications of Application

AEBSF hydrochloride is a broad spectrum, water soluble, irreversible serine protease inhibitor, TGF beta inhibitor, JNK activator, and p38 Map activator.

General Description

Pefabloc^? SC is a trypsin inhibitor, which can be used in the synthesis of its arginine-like analogue, 4-(2-guanidinoethyl)benzenesulfonyl fluoride.

Biochem/physiol Actions

Irreversible serine protease inhibitor. Inhibition constants are similar to those of PMSF and DFP. AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin kallikrein and thrombin. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water and improved stability in aqueous solutions. AEBSF has been used in cell culture in concentrations of up to 0.25?mM.

storage

Store at -20°C

References

1) Powers?et al. (2002),?Irreversible inhibitors of serine, cysteine and threonine proteases; Chem. Rev.,?102?4639 2) Diatchuk?et al.?(1997),?Inhibition of NADPH oxidase activation by 4-(2-aminoethyl)-benzenesulfonyl fluoride and related compounds; J. Biol. Chem.,?272?13292

Properties of 4-(2-Aminoethyl)benzenesulfonylfluoride hydrochloride

Melting point:	175-177 °C
storage temp.	2-8°C
solubility	H2O: soluble50mg/mL (stable for up to six months if stored refrigerated at a pH of less than 7. If a pH of greater than 7 is required, pH adjustment should be made just prior to use.)
form	Powder
color	White
PH	pH3.0～5.0 (10g/L, 25℃)
Water Solubility	10 g/L (20 ºC)
Sensitive	Moisture Sensitive
BRN	7604627
Stability:	Stable for 1 year from date of purchase as supplied. Solutions at pH <7 are stable for up to 6 months if stored in the refrigerator
CAS DataBase Reference	30827-99-7(CAS DataBase Reference)

Safety information for 4-(2-Aminoethyl)benzenesulfonylfluoride hydrochloride

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation
Precautionary Statement Codes	P264:Wash hands thoroughly after handling. P264:Wash skin thouroughly after handling. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. P332+P313:IF SKIN irritation occurs: Get medical advice/attention. P337+P313:IF eye irritation persists: Get medical advice/attention.