Bicinchoninic Acid Disodium Salt
Synonym(s):2,2′-Biquinoline-4,4′-dicarboxylic acid disodium salt;4,4′-Dicarboxy-2,2′-biquinoline disodium salt;BCA;Disodium 2,2′-biquinoline-4,4′-dicarboxylate
- CAS NO.:979-88-4
- Empirical Formula: C20H13N2NaO4
- Molecular Weight: 368.32
- MDL number: MFCD00037500
- EINECS: 629-761-2
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-10-29 11:48:30
What is Bicinchoninic Acid Disodium Salt ?
Description
2,2’-Biquinoline-4,4’-dicarboxylic acid (BCA) is a colorimetric reagent for the detection of copper (Cu+). BCA and Cu+ form a stable 2:1 complex that has an absorbance maximum at 562 nm. It is commonly used as a reagent in the colorimetric BCA protein quantitation assay in which Cu2+ is converted to Cu+ as a function of protein concentration under alkaline conditions.
Chemical properties
White to light yellow powder.
IUPAC Name is disodium [2,2'-biquinoline]-4,4'-dicarboxylate
The Uses of Bicinchoninic Acid Disodium Salt
For determination of Cu and protein assays.Bicinchoninic acid disodium salt is useful for the determination of copper. It is also used for the determination of protein concentration.
The Uses of Bicinchoninic Acid Disodium Salt
- Bicinchoninic acid disodium salt hydrate has been used to monitor the levels of reducing sugars, thereby helping in the detection of chitinases.
- It has also been used to determine the amount of cellulose reducing ends (micromoles/g cellulose).
- It has been used for determining protein concentration.
What are the applications of Application
Bicinchoninic acid disodium salt is a photometric reagent for protein assay
What are the applications of Application
2,2'-Bicinchoninic Acid Disodium Salt is a photometric reagent for protein assay. It is used for determination of reducing sugar and protein via Cu(I). Also a specific reagent for Cu(I) with sensitivity similar to that of neocuproin.
Biochem/physiol Actions
Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+), in presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.
Properties of Bicinchoninic Acid Disodium Salt
Melting point: | 300℃ |
storage temp. | Inert atmosphere,2-8°C |
solubility | 0.5% sodium bicarbonate: soluble50mg/mL |
form | Powder/Chunks |
color | white to light yellow |
Water Solubility | Soluble in water. |
Stability: | Hygrscopic |
InChI | InChI=1S/C20H12N2O4.Na.H/c23-19(24)13-9-17(21-15-7-3-1-5-11(13)15)18-10-14(20(25)26)12-6-2-4-8-16(12)22-18;;/h1-10H,(H,23,24)(H,25,26);; |
CAS DataBase Reference | 979-88-4(CAS DataBase Reference) |
EPA Substance Registry System | [2,2'-Biquinoline]-4,4'-dicarboxylic acid, disodium salt (979-88-4) |
Safety information for Bicinchoninic Acid Disodium Salt
Signal word | Warning |
Pictogram(s) |
Exclamation Mark Irritant GHS07 |
GHS Hazard Statements |
H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation |
Precautionary Statement Codes |
P264:Wash hands thoroughly after handling. P264:Wash skin thouroughly after handling. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. P332+P313:IF SKIN irritation occurs: Get medical advice/attention. P337+P313:IF eye irritation persists: Get medical advice/attention. |
Computed Descriptors for Bicinchoninic Acid Disodium Salt
InChIKey | AUPXFICLXPLHBB-UHFFFAOYSA-L |
SMILES | C(C1=CC(C2=NC3=CC=CC=C3C(C(=O)O)=C2)=NC2=CC=CC=C12)(=O)O.[NaH] |
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