Bicinchoninic Acid Disodium Salt

Synonym(s):2,2′-Biquinoline-4,4′-dicarboxylic acid disodium salt;4,4′-Dicarboxy-2,2′-biquinoline disodium salt;BCA;Disodium 2,2′-biquinoline-4,4′-dicarboxylate

CAS NO.：979-88-4
Empirical Formula: C20H13N2NaO4
Molecular Weight: 368.32
MDL number: MFCD00037500
EINECS: 629-761-2
SAFETY DATA SHEET (SDS)
Update Date: 2024-10-29 11:48:30

Bicinchoninic Acid Disodium Salt Structural

What is Bicinchoninic Acid Disodium Salt ?

Description

2,2’-Biquinoline-4,4’-dicarboxylic acid (BCA) is a colorimetric reagent for the detection of copper (Cu⁺). BCA and Cu⁺ form a stable 2:1 complex that has an absorbance maximum at 562 nm. It is commonly used as a reagent in the colorimetric BCA protein quantitation assay in which Cu²⁺ is converted to Cu⁺ as a function of protein concentration under alkaline conditions.

Chemical properties

White to light yellow powder.
IUPAC Name is disodium [2,2'-biquinoline]-4,4'-dicarboxylate

The Uses of Bicinchoninic Acid Disodium Salt

For determination of Cu and protein assays.Bicinchoninic acid disodium salt is useful for the determination of copper. It is also used for the determination of protein concentration.

The Uses of Bicinchoninic Acid Disodium Salt

Bicinchoninic acid disodium salt hydrate has been used to monitor the levels of reducing sugars, thereby helping in the detection of chitinases.
It has also been used to determine the amount of cellulose reducing ends (micromoles/g cellulose).
It has been used for determining protein concentration.

What are the applications of Application

Bicinchoninic acid disodium salt is a photometric reagent for protein assay

What are the applications of Application

2,2'-Bicinchoninic Acid Disodium Salt is a photometric reagent for protein assay. It is used for determination of reducing sugar and protein via Cu(I). Also a specific reagent for Cu(I) with sensitivity similar to that of neocuproin.

Biochem/physiol Actions

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+), in presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.

Properties of Bicinchoninic Acid Disodium Salt

Melting point:	300℃
storage temp.	Inert atmosphere,2-8°C
solubility	0.5% sodium bicarbonate: soluble50mg/mL
form	Powder/Chunks
color	white to light yellow
Water Solubility	Soluble in water.
Stability:	Hygrscopic
InChI	InChI=1S/C20H12N2O4.Na.H/c23-19(24)13-9-17(21-15-7-3-1-5-11(13)15)18-10-14(20(25)26)12-6-2-4-8-16(12)22-18;;/h1-10H,(H,23,24)(H,25,26);;
CAS DataBase Reference	979-88-4(CAS DataBase Reference)
EPA Substance Registry System	[2,2'-Biquinoline]-4,4'-dicarboxylic acid, disodium salt (979-88-4)

Safety information for Bicinchoninic Acid Disodium Salt

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation
Precautionary Statement Codes	P264:Wash hands thoroughly after handling. P264:Wash skin thouroughly after handling. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. P332+P313:IF SKIN irritation occurs: Get medical advice/attention. P337+P313:IF eye irritation persists: Get medical advice/attention.