N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic Acid

Synonym(s):2-[(2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)amino]ethanesulfonic acid;N-[Tris(hydroxymethyl)-methyl]-2-aminoethanesulfonic acid;N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid;TES;TES free acid

CAS NO.：7365-44-8
Empirical Formula: C6H15NO6S
Molecular Weight: 229.25
MDL number: MFCD00007532
EINECS: 230-906-3
SAFETY DATA SHEET (SDS)
Update Date: 2024-10-25 16:21:11

N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic Acid Structural

What is N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic Acid?

Chemical properties

White/clear crystalline powder

The Uses of N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic Acid

A structural analog to Tris buffer.

The Uses of N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic Acid

N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic Acid (TES) probably exists in solution as a zwitterion. Because the pKa is 7.4 (physiological pH) this buffer has considerable potential for a variety of biological applications. A buffer using TES free acid can be prepared by titrating the free acid (sc-206102) with sodium hydroxide to the desired pH, using about a half-equivalent of NaOH. Alternatively, solutions of equimolar TES free acid and TES sodium (sc-264384) can be mixed to attain a buffer of the desired pH. It has been shown to be toxic in vitro, but this toxicity may be due to its ability to form metal chelate complexes that are toxic for cells. TES is a commonly used biological buffering agent.

What are the applications of Application

TES, Free Acid is N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid, a structural analog to Tris buffer

Definition

ChEBI: N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid is a Good's buffer substance, pKa = 7.5 at 20 ℃. It is a member of ethanolamines, an amino sulfonic acid and a TES. It is functionally related to a taurine. It is a tautomer of a N-tris(hydroxymethyl)methyl-2-ammonioethanesulfonate.

Purification Methods

Crystallise the acid from hot EtOH containing a little water.

Properties of N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic Acid

Melting point:	~225 °C (dec.)
Density	1.260 (estimate)
refractive index	1.5500 (estimate)
storage temp.	Keep in dark place,Sealed in dry,Room Temperature
solubility	H₂O: 1 M at 20 °C, clear, colorless
form	Crystalline Powder
color	White
Odor	Odorless
PH	3.5-5.0 (25℃, 1M in H2O)
pka	7.5(at 25℃)
PH Range	6.8 - 8.2
Water Solubility	SOLUBLE
λmax	λ: 260 nm Amax: 0.055 λ: 280 nm Amax: 0.045
Merck	14,9176
BRN	1957061
CAS DataBase Reference	7365-44-8(CAS DataBase Reference)
EPA Substance Registry System	Ethanesulfonic acid, 2-[[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]amino]- (7365-44-8)

Safety information for N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic Acid

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection. P312:Call a POISON CENTER or doctor/physician if you feel unwell. P302+P352:IF ON SKIN: wash with plenty of soap and water. P304+P340:IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. P403+P233:Store in a well-ventilated place. Keep container tightly closed.