Thymine

Chemical properties

White, crystalline powder. Slightly soluble in hot water; insoluble in coldwater, alcohol; sparingly soluble in ether; readily soluble in alkalies.

The Uses of Thymine

Thymine is a nitrogenous base component in the nucleic acid of DNA.

The Uses of Thymine

A nucleobase found in deoxyribonucleic acids (DNA).

The Uses of Thymine

Thymine (Zidovudine EP Impurity C) is a nitrogenous base component in the nucleic acid of DNA.

What are the applications of Application

Thymine is a nucleobase found in deoxyribonucleic acids (DNA)

Definition

ChEBI: A pyrimidine nucleobase that is uracil in which the hydrogen at position 5 is replaced by a methyl group.

Definition

A nitrogenous base found in DNA. It has a PYRIMIDINE ring structure.

Definition

thymine: A pyrimidine derivativeand one of the major componentbases of nucleotides and the nucleicacid DNA.

Synthesis Reference(s)

The Journal of Organic Chemistry, 25, p. 149, 1960 DOI: 10.1021/jo01071a612

General Description

Thymine (Zidovudine Related Compound C) is a process related impurity of antiretroviral drug zidovudine. Zidovudine belongs to the class of drugs known as nucleoside reverse transcriptase inhibitors and is generally used in the prevention and treatment of HIV/AIDS.
Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.

Biochem/physiol Actions

Thymine used in studying DNA structure byirradtaion methods leading to cross-linking reactions and derivitizations. Thymine dimers are indicative of DNA damage. Thymine is used with metal (mercury) to form thymine?HgII?thymine (T?HgII?T) duplexes. Thymine starvation in bacteria leads to halt in DNA synthesis and is referred as thymine-less death.

Purification Methods

Crystallise thymine from EtOAc, 10% aqueous EtOH or water. It has m 318-320o after sublimation at 200o/12mm. Purify it by preparative (2mm thick) TLC plates of silica gel, eluting with ethyl acetate/isopropanol/water (75:16:9, v/v; RF 0.75). The desired spot is localated with a uv lamp, cut the band from the plate, place it in MeOH, shake and filter it through a millipore filter, then evaporate. [Infante et al. J Chem Soc, Faraday Trans 1 68 1586 1973, Beilstein 24 H 353, 24 I 330, 24 II 183, 24 III/IV 1292.]