SUC-LEU-LEU-VAL-TYR-AMC
- CAS NO.:94367-21-2
- Empirical Formula: C40H53N5O10
- Molecular Weight: 763.88
- MDL number: MFCD00080248
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-03-27 11:49:37
What is SUC-LEU-LEU-VAL-TYR-AMC?
Description
Suc-Leu-Leu-Val-Tyr-AMC (94367-21-2) is a fluorogenic substrate for the chymotryptic activity of the 20S proteasome1 and other chymotrypsin-like proteases, as well as calpains2. A commonly used substrate for assaying proteasomal enzymatic activity.3,4 Excitation max.: 360 nm; emission max.: 460 nm.
The Uses of SUC-LEU-LEU-VAL-TYR-AMC
Suc-Leu-Leu-Val-Tyr-AMC is a fluorgenic substrate for chymotrypsin-like proteases, as well as calpains. It can be used to treat tuberculosis.
Definition
ChEBI: A tetrapeptide compound with a succinyl group at the N-terminal and a 7-amino-4-methylcoumarin group at the C-terminal.
Biochem/physiol Actions
In the presence of chymotrypsin-like enzyme activity, the fluorophore, 7-amido-4-methylcoumarin is released from N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin. The fluorescence obtained is a measure of the enzyme activity.6
References
1) Stein et al. (1996), Kinetic characterization of the chymotryptic activity of the 20S proteasome; Biochemistry, 35 3899 2) Sasaki et al. (1984), Comparative specificity and kinetic studies on porcine calpain I and calpain II with naturally occurring peptides and synthetic fluorogenic substrates; J. Biol. Chem., 259 12489 3) Hamouda et al. (2014), The small heat shock protein B8 (HSPB8) confers resistance to bortezomib by promoting autophagic removal of misfolded proteins in multiple myeloma cells; Oncotarget, 5 6252 4) Min et al. (2017), USP14 inhibitor attenuates cerebral ischemia/reperfusion-induced neuronal injury in mice; J. Neurochem,, 140 826
Properties of SUC-LEU-LEU-VAL-TYR-AMC
Boiling point: | 1116.8±65.0 °C(Predicted) |
Density | 1.249±0.06 g/cm3(Predicted) |
storage temp. | -20°C |
solubility | Soluble in DMSO (up to 20 mg/ml), or in DMF (up to 10 mg/ml) |
form | Lyophilized solid |
pka | 4.69±0.10(Predicted) |
color | White to Off-White |
Stability: | Stable for 1 year from date of purchase as supplied. Solutions in DMSO or DMF may be stored at -20°C for up to 1 month. |
Safety information for SUC-LEU-LEU-VAL-TYR-AMC
GHS Hazard Statements |
H413:Hazardous to the aquatic environment, long-term hazard |
Precautionary Statement Codes |
P273:Avoid release to the environment. |
Computed Descriptors for SUC-LEU-LEU-VAL-TYR-AMC
New Products
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