PYRONIN Y

Synonym(s):(C.I. 45005) for microscopy Certistain;Pyronin G;Pyronin J;Pyronin Y;Pyronine G (C.I. 45005)

CAS NO.：92-32-0
Empirical Formula: C17H19ClN2O
Molecular Weight: 302.8
MDL number: MFCD00011725
EINECS: 202-147-8
SAFETY DATA SHEET (SDS)
Update Date: 2024-11-19 15:53:33

What is PYRONIN Y?

Chemical properties

green crystalline solid

The Uses of PYRONIN Y

Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.

The Uses of PYRONIN Y

Bacterial and biological stain.

The Uses of PYRONIN Y

Pyronin Y has been used:

in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)
as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)
staining of CD34⁺ cells

What are the applications of Application

Pyronin Y is a dye that intercalates and stains RNA red

Definition

ChEBI: An organic chloride salt having 6-(dimethylamino)-N,N-dimethyl-3H-xanthen-3-iminium as the cation. Used with methyl green to selectively demonstrate RNA (red) in contrast to DNA (green) with the Unna Pappenheim method.

General Description

Pyronin Y (C.I. 45005) is an aminoxanthene dye that is present as a weakly lipophilic cation in neutral aqueous solutions. It is also known as pyronine G.

Biochem/physiol Actions

Pyronin Y is important in histochemistry and is combined with methyl green for staining of RNA. The mixture results in bright red/green staining of nucleic acids.

storage

Room temperature

Purification Methods

Commercial material may contain a large quantity of zinc. Purify it by dissolving 1g in 50mL of hot water containing 5g NaEDTA. Cool to 0o, filter, evaporate to dryness and the residue is extracted with EtOH. The solution is evaporated to 5-10mL, filtered, and the dye is precipitated by addition of excess of dry diethyl ether. It is centrifuged, and the crystals are washed with dry ether. The procedure is repeated, then the product is dissolved in CHCl3, filtered and evaporated. The dye is stored in a vacuum. [Beilstein 18 H 596, 18 III/IV 7361, 18/10 V 181.]

Properties of PYRONIN Y

Melting point:	250-260 °C(lit.)
storage temp.	room temp
solubility	Soluble in water; sparingly soluble in ethanol, ethylene glycol, methyl cellosolve
form	Powder/Solid
Colour Index	45005
color	Pink
Water Solubility	Soluble in water. Also soluble in methyl cellosolve (9 mg/mL) and ethanol (3 mg/mL).
λmax	548 nm
Merck	13,8097
BRN	3795789
Stability:	Stable. Incompatible with strong oxidizing agents.
Biological Applications	Antimalarial agent; antiviral agent; nucleic acid sequencing; treating protozoan infections; herbicides
CAS DataBase Reference	92-32-0
EPA Substance Registry System	Pyronine Y (92-32-0)

Safety information for PYRONIN Y

Signal word	Danger
Pictogram(s)	Health Hazard GHS08
Precautionary Statement Codes	P201:Obtain special instructions before use. P202:Do not handle until all safety precautions have been read and understood. P281:Use personal protective equipment as required. P308+P313:IF exposed or concerned: Get medical advice/attention. P403:Store in a well-ventilated place.