Contact us: +91 9550333722 040 - 40102781
Structured search
India
Choose your country
Different countries will display different contents
Try our best to find the right business for you.
My chemicalbook

Welcome back!

HomeProduct name listPUROMYCIN DIHYDROCHLORIDE

PUROMYCIN DIHYDROCHLORIDE

Synonym(s):(2 S)-2-Amino-N-((2 S,3 S,4 R,5 R)-5-(6-(dimethylamino)purin-9-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl)-3-(4-methoxyphenyl);3′-[α-Amino-p-methoxyhydrocinnamamido]-3′-deoxy-N,N-dimethyladenosine dihydrochloride;Puromycin, Dihydrochloride, Sterile-Filtered Aqueous Solution, Cell Culture-Tested - CAS 58-58-2 - Calbiochem

  • CAS NO.:58-58-2
  • Empirical Formula: C22H30ClN7O5
  • Molecular Weight: 507.98
  • MDL number: MFCD00012691
  • EINECS: 200-387-8
  • SAFETY DATA SHEET (SDS)
  • Update Date: 2024-11-07 21:39:59
PUROMYCIN DIHYDROCHLORIDE Structural

What is PUROMYCIN DIHYDROCHLORIDE?

Description

Puromycin (58-58-2) is an aminonucleoside antibiotic derived from Streptomyces alboniger. It is a protein synthesis inhibitor that disrupts peptide transfer on ribosomes causing premature chain termination.1 It is an inhibitor of dipeptidyl-peptidase II and cytosolic alanyl aminopeptidase.2 Puromycin also inhibits protein transport in mitochondria.3

Chemical properties

white powder

The Uses of PUROMYCIN DIHYDROCHLORIDE

Puromycin dihydrochloride is the salt of puromycin, a nucleoside antibiotic isolated from Streptomyces alboniger in the 1950s as an anti-trypansomal agent with antibiotic activity. While the salt shares the same pharmacological properties as puromycin free base, its greater water solubility offers advantages in some in vitro applications. Physicochemical properties and chromatographic behaviour will depend on whether the pH is buffered. In non-pH controlled systems, the free base and salt may behave differently.

The Uses of PUROMYCIN DIHYDROCHLORIDE

Puromycin is an aminonucleoside antibitotic. Puromycin acts as a protein synthesis inhibitor which interferes with translation by inhibiting ribosome functions. Puromycin is also a reversible inhibitor of dipeptidyl-peptidase II (serine peptidase) and cytosol alanyl aminopeptidase.

The Uses of PUROMYCIN DIHYDROCHLORIDE

antibacterial

The Uses of PUROMYCIN DIHYDROCHLORIDE

Puromycin dihydrochloride is a salt of puromycin, a nucleoside antibiotic isolated from Streptomyces alboniger in the 1950s as an anti-trypansomal agent with antibiotic activity. While the salt shares the same pharmacological properties as puromycin free base, its greater water solubility offers advantages in some in vitro applications. Physicochemical properties and chromatographic behaviour will depend on whether the pH is buffered. In non-pH controlled systems, the free base and salt may behave differently.

The Uses of PUROMYCIN DIHYDROCHLORIDE

An aminonuclease antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene.

The Uses of PUROMYCIN DIHYDROCHLORIDE

Research tool for studying protein synthesis; cell line selection agent in gene transfer experiments.

What are the applications of Application

Puromycin dihydrochloride is an antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene

General Description

White powder.

Air & Water Reactions

Water soluble.

Reactivity Profile

PUROMYCIN DIHYDROCHLORIDE is sensitive to prolonged exposure to heat. . Behaves as a very weak acid in solution.

Fire Hazard

Flash point data for PUROMYCIN DIHYDROCHLORIDE are not available, but PUROMYCIN DIHYDROCHLORIDE is probably combustible.

storage

-20°C

Purification Methods

Puromycin dihydrochloride is purified by recrystallisation from H2O. The free base, [58-60-6] M 294.3, has m 175.5-177o (172-173o) (from H2O). The sulfate has m 180-187o dec (from H2O), and the picrate monohydrate has m 146-149o (from H2O). [Baker et al. J Am Chem Soc 77 1 1955, Fryth et al. J Am Chem Soc 80 3736 1958.] It is an inhibitor of aminopeptidase and terminates protein synthesis [Reboud et al. Biochemistry 20 5281 1981]. [Beilstein 26 III/IV 3704.]

References

1) Azzam and Algranati; (1973) Mechanism of puromycin action: fate of ribosomes after release of nascent polypeptide chains from polysomes; Proc. Nat. Acad. Sci. USA, 70 3866 2) Bhutani et al. (2007) Puromycin-sensitive aminopeptidase is the major peptidase responsible for digesting polyglutamine sequences by proteasomes during protein degradation; EMBO J., 26 1385 3) Price and Verner (1993) Puromycin inhibits protein import into mitochondria by interfering with an intramitochondrial ATP-dependent reaction; Biochim. Biophys. Acta, 1150 89

Properties of PUROMYCIN DIHYDROCHLORIDE

Melting point: 168-170℃
storage temp.  Inert atmosphere,Store in freezer, under -20°C
solubility  H2O: soluble50mg/mL (Sterilize stock solution by filtration using 0.22 μm filter then store in aliquots at 20 °C.)
form  solution
color  yellow-white
Water Solubility  Soluble in water
Merck  14,7943
BRN  3853613
Stability: Stable. Heat sensitive. Incompatible with strong oxidizing agents.
EPA Substance Registry System Adenosine, 3'-[[(2S)-2-amino-3-(4-methoxyphenyl)-1-oxopropyl]amino]-3'-deoxy-N,N-dimethyl-, dihydrochloride (58-58-2)

Safety information for PUROMYCIN DIHYDROCHLORIDE

Signal word Warning
Pictogram(s)
ghs
Exclamation Mark
Irritant
GHS07
GHS Hazard Statements H302:Acute toxicity,oral

Computed Descriptors for PUROMYCIN DIHYDROCHLORIDE

InChIKey MKSVFGKWZLUTTO-NGOAXYITSA-N

Related products of tetrahydrofuran

You may like

  • Puromycin Dihydrochloride (PRM) for cell culture CAS 58-58-2
    Puromycin Dihydrochloride (PRM) for cell culture CAS 58-58-2
    58-58-2
    View Details
  • Puromycine dihyrdrochloride CAS 58-58-2
    Puromycine dihyrdrochloride CAS 58-58-2
    58-58-2
    View Details
  • Puromycin Dihydrochloride (PRM) CAS 58-58-2
    Puromycin Dihydrochloride (PRM) CAS 58-58-2
    58-58-2
    View Details
  • Puromycin ready made solution CAS
    Puromycin ready made solution CAS
    View Details
  • Puromycin dihydrochloride from Streptomyces alboniger CAS 58-58-2
    Puromycin dihydrochloride from Streptomyces alboniger CAS 58-58-2
    58-58-2
    View Details
  • Puromycin dihydrochloride CAS 58-58-2
    Puromycin dihydrochloride CAS 58-58-2
    58-58-2
    View Details
  • Puromycin dihydrochloride from Streptomyces alboniger CAS 58-58-2
    Puromycin dihydrochloride from Streptomyces alboniger CAS 58-58-2
    58-58-2
    View Details
  • Edoxaban Impurity 57
    Edoxaban Impurity 57
    2089454-69-1
    View Details
Statement: All products displayed on this website are only used for non medical purposes such as industrial applications or scientific research, and cannot be used for clinical diagnosis or treatment of humans or animals. They are not medicinal or edible.