PUROMYCIN DIHYDROCHLORIDE
Synonym(s):(2 S)-2-Amino-N-((2 S,3 S,4 R,5 R)-5-(6-(dimethylamino)purin-9-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl)-3-(4-methoxyphenyl);3′-[α-Amino-p-methoxyhydrocinnamamido]-3′-deoxy-N,N-dimethyladenosine dihydrochloride;Puromycin, Dihydrochloride, Sterile-Filtered Aqueous Solution, Cell Culture-Tested - CAS 58-58-2 - Calbiochem
- CAS NO.:58-58-2
- Empirical Formula: C22H30ClN7O5
- Molecular Weight: 507.98
- MDL number: MFCD00012691
- EINECS: 200-387-8
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-11-19 20:33:22
What is PUROMYCIN DIHYDROCHLORIDE?
Description
Puromycin (58-58-2) is an aminonucleoside antibiotic derived from Streptomyces alboniger. It is a protein synthesis inhibitor that disrupts peptide transfer on ribosomes causing premature chain termination.1 It is an inhibitor of dipeptidyl-peptidase II and cytosolic alanyl aminopeptidase.2 Puromycin also inhibits protein transport in mitochondria.3
Chemical properties
white powder
The Uses of PUROMYCIN DIHYDROCHLORIDE
Puromycin dihydrochloride is the salt of puromycin, a nucleoside antibiotic isolated from Streptomyces alboniger in the 1950s as an anti-trypansomal agent with antibiotic activity. While the salt shares the same pharmacological properties as puromycin free base, its greater water solubility offers advantages in some in vitro applications. Physicochemical properties and chromatographic behaviour will depend on whether the pH is buffered. In non-pH controlled systems, the free base and salt may behave differently.
The Uses of PUROMYCIN DIHYDROCHLORIDE
Puromycin is an aminonucleoside antibitotic. Puromycin acts as a protein synthesis inhibitor which interferes with translation by inhibiting ribosome functions. Puromycin is also a reversible inhibitor of dipeptidyl-peptidase II (serine peptidase) and cytosol alanyl aminopeptidase.
The Uses of PUROMYCIN DIHYDROCHLORIDE
antibacterial
The Uses of PUROMYCIN DIHYDROCHLORIDE
Puromycin dihydrochloride is a salt of puromycin, a nucleoside antibiotic isolated from Streptomyces alboniger in the 1950s as an anti-trypansomal agent with antibiotic activity. While the salt shares the same pharmacological properties as puromycin free base, its greater water solubility offers advantages in some in vitro applications. Physicochemical properties and chromatographic behaviour will depend on whether the pH is buffered. In non-pH controlled systems, the free base and salt may behave differently.
The Uses of PUROMYCIN DIHYDROCHLORIDE
An aminonuclease antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene.
The Uses of PUROMYCIN DIHYDROCHLORIDE
Research tool for studying protein synthesis; cell line selection agent in gene transfer experiments.
What are the applications of Application
Puromycin dihydrochloride is an antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene
General Description
White powder.
Air & Water Reactions
Water soluble.
Reactivity Profile
PUROMYCIN DIHYDROCHLORIDE is sensitive to prolonged exposure to heat. . Behaves as a very weak acid in solution.
Fire Hazard
Flash point data for PUROMYCIN DIHYDROCHLORIDE are not available, but PUROMYCIN DIHYDROCHLORIDE is probably combustible.
storage
-20°C
Purification Methods
Puromycin dihydrochloride is purified by recrystallisation from H2O. The free base, [58-60-6] M 294.3, has m 175.5-177o (172-173o) (from H2O). The sulfate has m 180-187o dec (from H2O), and the picrate monohydrate has m 146-149o (from H2O). [Baker et al. J Am Chem Soc 77 1 1955, Fryth et al. J Am Chem Soc 80 3736 1958.] It is an inhibitor of aminopeptidase and terminates protein synthesis [Reboud et al. Biochemistry 20 5281 1981]. [Beilstein 26 III/IV 3704.]
References
1) Azzam and Algranati; (1973) Mechanism of puromycin action: fate of ribosomes after release of nascent polypeptide chains from polysomes; Proc. Nat. Acad. Sci. USA, 70 3866 2) Bhutani et al. (2007) Puromycin-sensitive aminopeptidase is the major peptidase responsible for digesting polyglutamine sequences by proteasomes during protein degradation; EMBO J., 26 1385 3) Price and Verner (1993) Puromycin inhibits protein import into mitochondria by interfering with an intramitochondrial ATP-dependent reaction; Biochim. Biophys. Acta, 1150 89
Properties of PUROMYCIN DIHYDROCHLORIDE
Melting point: | 168-170℃ |
storage temp. | Inert atmosphere,Store in freezer, under -20°C |
solubility | H2O: soluble50mg/mL (Sterilize stock solution by filtration using 0.22 μm filter then store in aliquots at 20 °C.) |
form | solution |
color | yellow-white |
Water Solubility | Soluble in water |
Merck | 14,7943 |
BRN | 3853613 |
Stability: | Stable. Heat sensitive. Incompatible with strong oxidizing agents. |
EPA Substance Registry System | Adenosine, 3'-[[(2S)-2-amino-3-(4-methoxyphenyl)-1-oxopropyl]amino]-3'-deoxy-N,N-dimethyl-, dihydrochloride (58-58-2) |
Safety information for PUROMYCIN DIHYDROCHLORIDE
Signal word | Warning |
Pictogram(s) |
Exclamation Mark Irritant GHS07 |
GHS Hazard Statements |
H302:Acute toxicity,oral |
Computed Descriptors for PUROMYCIN DIHYDROCHLORIDE
InChIKey | MKSVFGKWZLUTTO-NGOAXYITSA-N |
Abamectin manufacturer
Anand Agencies
New Products
4-Aminotetrahydropyran-4-carbonitrile Hydrochloride (R)-3-Aminobutanenitrile Hydrochloride 4-AMINO-TETRAHYDRO-PYRAN-4-CARBOXYLIC ACID HCL 4-(Dimethylamino)tetrahydro-2H-pyran-4-carbonitrile 3-((Dimethylamino)methyl)-5-methylhexan-2-one oxalate 1,4-Dioxa-8-azaspiro[4.5]decane 5-Bromo-2-nitropyridine Nimesulide BP Aceclofenac IP/BP/EP Diclofenac Sodium IP/BP/EP/USP Mefenamic Acid IP/BP/EP/USP Ornidazole IP Diclofenac Potassium SODIUM AAS SOLUTION ZINC AAS SOLUTION BUFFER SOLUTION PH 10.0(BORATE) GOOCH CRUCIBLE SINTERED AQUANIL 5 BERYLLIUM AAS SOLUTION 2-Bromo-1-(bromomethyl)-3-chloro-5-nitrobenzene 2-Bromo-3-nitroaniline N-(3-Hydroxypropyl)-N-methylacetamide 3-Bromo-6-chloropyridazine 4-ethyl-3-nitrobenzoic acidRelated products of tetrahydrofuran
You may like
-
Puromycin Dihydrochloride (PRM) for cell culture CAS 58-58-2View Details
58-58-2 -
Puromycine dihyrdrochloride CAS 58-58-2View Details
58-58-2 -
Puromycin Dihydrochloride (PRM) CAS 58-58-2View Details
58-58-2 -
Puromycin ready made solution CASView Details
-
Puromycin dihydrochloride from Streptomyces alboniger CAS 58-58-2View Details
58-58-2 -
Puromycin dihydrochloride CAS 58-58-2View Details
58-58-2 -
Puromycin dihydrochloride from Streptomyces alboniger CAS 58-58-2View Details
58-58-2 -
Lithium ClavulanateView Details
61177-44-4