N-METHOXYSUCCINYL-ALA-ALA-PRO-VAL P-NITROANILIDE

Synonym(s):Elastase Substrate I, Colorimetric - CAS 70967-90-7 - Calbiochem;Methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide - CAS 70967-90-7 - Calbiochem;N-(Methoxysuccinyl)-L-alanyl-L-alanyl-L-prolyl-L-valine 4-nitroanilide;N-(Methoxysuccinyl)-Ala-Ala-Pro-Val 4-nitroanilide

CAS NO.：70967-90-7
Empirical Formula: C27H38N6O9
Molecular Weight: 590.63
MDL number: MFCD00077137
EINECS: 232-670-7
SAFETY DATA SHEET (SDS)
Update Date: 2024-10-28 16:48:35

N-METHOXYSUCCINYL-ALA-ALA-PRO-VAL P-NITROANILIDE Structural

What is N-METHOXYSUCCINYL-ALA-ALA-PRO-VAL P-NITROANILIDE?

Description

Proteinase 3 (PR3, myeloblastin) is a polymorphonuclear leukocyte serine proteinase that degrades matrix proteins including fibronectin, laminin, vitronectin, and collagen type IV to generate antimicrobial peptides. Neutrophil elastase is a serine proteinase that is secreted by neutrophils during inflammation to destroy pathogens. Evaluating these enzymes is helpful to understanding inflammatory autoimmune processes. MeOSuc-AAPV-pNA is a highly sensitive peptide substrate that is hydrolyzed by both human and mouse neutrophil elastase and PR3, but not cathepsin G or chymotrypsin. Enzyme activity can be quantified by colorimetric detection of free p-nitroanilide at 405 nm.

The Uses of N-METHOXYSUCCINYL-ALA-ALA-PRO-VAL P-NITROANILIDE

N-Methoxysuccinyl-Ala-Ala-Pro-Val p-Nitroanilide is a highly sensitive peptide substrate that is hydrolyzed by both human and mouse neutrophil elastase and PR3 but not cathespin.

What are the applications of Application

MeOSuc-AAPV-pNA, Chromogenic Substrate is a sensitive PR3 and neutrophil elastase chromogenic substrate

Properties of N-METHOXYSUCCINYL-ALA-ALA-PRO-VAL P-NITROANILIDE

Melting point:	203-205°C
Boiling point:	958.4±65.0 °C(Predicted)
Density	1.297±0.06 g/cm3(Predicted)
storage temp.	-20°C
solubility	DMF: 25 mg/mL, clear, light yellow
form	Off-white solid
pka	13.29±0.20(Predicted)
color	White to Off-White
BRN	6266359