FDG

Synonym(s):FDG

CAS NO.：17817-20-8
Empirical Formula: C32H32O15
Molecular Weight: 656.59
MDL number: MFCD00077344
EINECS: 1312995-182-4
SAFETY DATA SHEET (SDS)
Update Date: 2025-12-16 21:30:20

What is FDG?

The Uses of FDG

For detecting Glycosidases and could be used in ELISAs, FDG substrate has also been used for identifying lacZ-positive cells with fluorescence microscopy and flow cytometry

The Uses of FDG

Ultra sensitive fluorogenic substrate for ?-galactosidase, used in live cell and tissue (in vivo and in vitro) assays. This fluorescent substrate reagent could be used for monitoring kinetics of hydrolysis.

The Uses of FDG

Fluorescein di(β-D-galactopyranoside) (FDG) has been used for the detection of gene expression via FACS (fluorescence-activated cell sorting).

What are the applications of Application

Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase

Biochem/physiol Actions

Fluorescein di (β-D-galactopyranoside) (FDG) results in fluorescence after being hydrolyzed to fluorescein by β-galactosidase. It gives fluorescence proportional to the enzymatic activity. This molecule is mainly required for the detection of gene expression in transfected cells when using β-galactosidase (Lac Z) as a reporter gene.

Properties of FDG

Melting point:	200-203 °C (dec.)
Boiling point:	974.8±65.0 °C(Predicted)
Density	1.74±0.1 g/cm3(Predicted)
storage temp.	-20°C
solubility	DMSO: soluble
form	A solid
pka	12.44±0.70(Predicted)
color	Yellow to orange

Safety information for FDG

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P280:Wear protective gloves/protective clothing/eye protection/face protection.