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HomeProduct name listEC 3.4.21.14

EC 3.4.21.14

Synonym(s):Alcalase 2.4L;Esperase 8.0L;Everlase 16.0 L;Proteinase from Bacillus licheniformis;Savinase 16.0L

  • CAS NO.:9014-01-1
  • Empirical Formula: Unspecified
  • Molecular Weight: 0
  • MDL number: MFCD00165548
  • EINECS: 232-752-2
  • SAFETY DATA SHEET (SDS)
  • Update Date: 2024-11-01 18:09:03

What is EC 3.4.21.14?

Chemical properties

These are proteolytic enzymes which take the form of light-colored, free-flowing powders. A protein containing numerous amino acids

The Uses of EC 3.4.21.14

This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is for use in Total Dietary Fiber Assays (TDF-100A).

The Uses of EC 3.4.21.14

Protease from Bacillus licheniformis has been used to digest the neurolemma in the antennal lobe of the moth; Heliothis virescens.

The Uses of EC 3.4.21.14

Protease from Bacillus sp. has been used to determine its proteolytic activity by spectrophotometric method.

General Description

Proteolytic enzymes are known to possess catalytic, non-catalytic and ancillary domains. Proteases are broadly classified as endopeptidases and exopeptidases. Functionally they are divided as aspartic, glutamic, cysteine, threonine, serine and metalloproteases.

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Proteases, secreted from Bacillus sp., typically have molecular weights ranging from 20,000 to 30,000. They are typcially stabilized by Ca2+ and have high isoelectric points.

Safety Profile

Moderately toxic by ingestion. Aneye irritant. When heated to decomposition it emits toxicfumes of NOx.

Potential Exposure

These commercial proteolytic enzymes are used in laundry detergent formulations

Purification Methods

This alkaline protease is purified 211-fold by affinity chromatography using 4-(4-aminophenylazo)phenylarsonic acid complex to activated CH-Sepharose 4B. It is inhibited by 2-phenylethane boronic acid, PMSF, 3,4-dichloroisocoumarin, acetone and benzamide. [Chandraskaren & Dhar Anal Biochem 150 141 1985, Schomburg & Schomburg Springer Handbook of Enzymes 2nd Edn vol 7 p 286 2002.] Synexin (from bovine liver) M 47,000 Da. This Ca binding protein is purified by (NH4)2SO4 precipitation, then by a specific pH step elution from a chromatofocusing medium in the absence of ampholytes. The pI is 7.5. [Scott et al. Anal Biochem 149 163 1985.]

Incompatibilities

Incompatible with oxidizers (chlorates, nitrates, peroxides, permanganates, perchlorates, chlorine, bromine, fluorine, etc.); contact may cause fires or explosions. Keep away from alkaline materials, strong bases, strong acids, oxoacids, epoxides.

Properties of EC 3.4.21.14

Density  1.3
vapor pressure  0Pa at 25℃
storage temp.  2-8°C
form  powder
color  white
Water Solubility  100g/L at 25℃
Stability: store cold
CAS DataBase Reference 9014-01-1
EPA Substance Registry System Subtilisin (9014-01-1)

Safety information for EC 3.4.21.14

Signal word Danger
Pictogram(s)
ghs
Corrosion
Corrosives
GHS05
ghs
Exclamation Mark
Irritant
GHS07
ghs
Health Hazard
GHS08
ghs
Environment
GHS09
GHS Hazard Statements H302:Acute toxicity,oral
H315:Skin corrosion/irritation
H318:Serious eye damage/eye irritation
H334:Sensitisation, respiratory
H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
H410:Hazardous to the aquatic environment, long-term hazard
Precautionary Statement Codes P261:Avoid breathing dust/fume/gas/mist/vapours/spray.
P273:Avoid release to the environment.
P280:Wear protective gloves/protective clothing/eye protection/face protection.
P301+P312:IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.
P302+P352:IF ON SKIN: wash with plenty of soap and water.
P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

Computed Descriptors for EC 3.4.21.14

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