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HomeProduct name listDIHYDRORHODAMINE 123

DIHYDRORHODAMINE 123

Synonym(s):Dihydrorhodamine 123 - CAS 109244-58-8 - Calbiochem

DIHYDRORHODAMINE 123 Structural

What is DIHYDRORHODAMINE 123?

Description

DHR is a cell-permeable fluorogenic probe that is used as an indicator of intracellular peroxynitrite formation. It is oxidized by peroxynitrite to the highly fluorescent product rhodamine in vitro. Neither nitric oxide, superoxide, nor hydrogen peroxide alone appear to oxidize DHR. Formation of rhodamine can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 500 and 536 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 78,800 M−1cm−1). DHR has been used to investigate reactive oxygen intermediates produced by endothelial cells, eosinophils, and reactive microglia.

The Uses of DIHYDRORHODAMINE 123

Dihydrorhodamine 123 is the uncharged and nonfluorescent reduction product of the mitochondrion-selective dye rhodamine 123 used for investigation of reacitve oxygen intermediates.

The Uses of DIHYDRORHODAMINE 123

Dihydrorhodamine 123 (DHR) has been used for the detection of free intracellular radicals or ROS (reactive oxygen species) in head kidney-derived macrophages. It has also been used in DHR assay in red blood cells and in oxidative burst assay in neutrophils.

What are the applications of Application

Dihydrorhodamine 123 is a fluorescent probe commonly used for determination of peroxynitrite & other ROSs

General Description

Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) such as peroxide and peroxynitrite. Dihydrorhodamine is not fluorescent unless it is converted to rhodamine 123.

Biochem/physiol Actions

Cell permeable: yes

Properties of DIHYDRORHODAMINE 123

Melting point: 163–165℃
Boiling point: 526.9±50.0 °C(Predicted)
Density  1.313±0.06 g/cm3(Predicted)
storage temp.  -20°C
solubility  DMSO: >5mg/mL
pka 4.70 ± 0.40, most basic, temperature: 25 °C
form  powder
color  Light red or pinkish-white
λmax 289 nm
BRN  5989965
Biological Applications Generating,detecting & measuring reactive oxygen species; generating,detecting & measuring reactive nitrogen species; detecting phosphates,peroxynitrile; measuring respiratory burst; chronic granulomatous disease DHR assay; screening

Safety information for DIHYDRORHODAMINE 123

Computed Descriptors for DIHYDRORHODAMINE 123

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