Desthiobiotin-PEG4-Alkyne
- CAS NO.:1802907-99-8
- Empirical Formula: C21H37N3O6
- Molecular Weight: 427.54
- MDL number: MFCD28505545
- Update Date: 2024-03-21 17:31:59
What is Desthiobiotin-PEG4-Alkyne?
Description
Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.
Properties of Desthiobiotin-PEG4-Alkyne
Boiling point: | 649.0±50.0 °C(Predicted) |
Density | 1.075±0.06 g/cm3(Predicted) |
pka | 14.93±0.60(Predicted) |
Safety information for Desthiobiotin-PEG4-Alkyne
Computed Descriptors for Desthiobiotin-PEG4-Alkyne
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