Dansyl chloride

Chemical properties

yellow to orange-yellow crystalline powder

The Uses of Dansyl chloride

Reagent for fluorescent labelling of amines, amino acids and proteins.

The Uses of Dansyl chloride

A fluorogenic reagent for N-terminal derivatization of amino acids and peptides and detection by reverse phase HPLC.

The Uses of Dansyl chloride

In fluorescent labelling, column chromatography, HPLC.

The Uses of Dansyl chloride

Dansyl chloride is a fluorogenic reagent used for protein, N-terminal amino acid and peptide detection by reverse phase high performance liquid chromatography (HPLC). It is also used to determine the degree of labeling in chymotrypsin and ovalbumin. It acts as a precursor for the synthesis of other derivatives like dansyl amide.

Definition

ChEBI: Dansyl chloride is an aminonaphthalene and a sulfonic acid derivative.

General Description

Dansyl chloride is a fluorogenic reagent which is typically employed for the derivatization of amino acids.

Biochem/physiol Actions

Dansyl chloride is a sulfonyl chloride compound. It is used to end tag amino groups of peptides and proteins. Dansyl chloride is used for derivatization of free amino acids and amines in neural samples and biological fluids. It is used to study fluorescent conjugates of albumin, protein conformational changes studies and active site characterization.

Safety Profile

Poison by intravenous route, When heated to decomposition it emits very toxic fumes of Cland NOx