Aniline Blue

Synonym(s):Aniline Blue W.S.;Blackley Blue

CAS NO.：28631-66-5
Empirical Formula: C32H27N3O9S3.2Na
Molecular Weight: 737.73
MDL number: MFCD00036130
EINECS: 249-113-9
SAFETY DATA SHEET (SDS)
Update Date: 2024-03-08 19:21:35

What is Aniline Blue?

Chemical properties

Aniline Blue is a dark brownish-purple crystalline powder. It is soluble and used as a biological stain in fluorescent microscopy, appearing yellow-green after excitation with violet light.

The Uses of Aniline Blue

Aniline Blue is a biological stain used for staining collagen

Definition

ChEBI: Aniline Blue is an organic sodium salt resulting from the formal condensation of NSC 56820 with 2 mol eq. of sodium hydroxide. It is used as a histological stain for collagen and in Mallory's method for connective tissue.

Preparation

C.I. Solvent Blue 3 (C.I. 42775) with strong sulfuric acid three stlfonation, and translated into sodium salt.

Properties and Applications

blue. Soluble in cold water and hot water (blue), slightly soluble in alcohol. Meet strong sulfuric acid as red light yellow, diluted into blue, and produce blue precipitation. Its water solution and sodium hydroxide is palm red light. For wool, silk, cotton, paper, leather dyeing. Used for plastic ones coloring of casein, also used in the manufacture of ink. The salt can be used as organic pigments used in rubber color.

Properties of Aniline Blue

storage temp.	Store at +15°C to +30°C.
form	Powder/Solid
color	Dark brown-purple crystal with metal luster
EPA Substance Registry System	C.I. Acid Blue 22 (28631-66-5)

Safety information for Aniline Blue

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P264:Wash hands thoroughly after handling. P264:Wash skin thouroughly after handling. P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.