AC-YVAD-AFC

Synonym(s):Ac-YVAD-AFC;Caspase-1 Substrate VI, Fluorogenic - CAS 219137-85-6 - Calbiochem;Z-YVAD-AFC

CAS NO.：219137-85-6
Empirical Formula: C33H36F3N5O10
Molecular Weight: 719.66
MDL number: MFCD01318881
SAFETY DATA SHEET (SDS)
Update Date: 2023-05-04 17:34:35

What is AC-YVAD-AFC?

Description

Ac-YVAD-AFC is a substrate whose amino acids YVAD have been shown to be a preferred cleavage site for caspase-1 and -4. Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm.

The Uses of AC-YVAD-AFC

N-Acetyl-Tyr-Val-Ala-Asp-7-amido-4-trifluoromethylcoumarin is a substrate whose amino acids are shown as a preferred cleavage site for Caspase-1 and 4. Useful for caspase research.

What are the applications of Application

Ac-YVAD-AFC is a fluorogenic substrate for caspase-1 and caspase-4

Definition

ChEBI: Ac-Tyr-Val-Ala-Asp-7-amino-4-trifluoromethylcoumarin is a tetrapeptide obtained by formal condensation of the C-terminal carboxy group of Ac-Tyr-Val-Ala-Asp with the amino group of 7-amino-4-trifluoromethylcoumarin. It has a role as a protease inhibitor. It is a tetrapeptide, a member of acetamides, a member of coumarins and an organofluorine compound. It is functionally related to a 7-amino-4-(trifluoromethyl)coumarin.

Biological Activity

Fluorogenic peptide substrate for caspase-1 (ICE).

Properties of AC-YVAD-AFC

Melting point:	>190°C (dec.)
Boiling point:	1084.6±65.0 °C(Predicted)
Density	1.416±0.06 g/cm3(Predicted)
storage temp.	−20°C
solubility	DMSO (Slightly), Methanol (Silghtly)
form	powder
pka	4.09±0.10(Predicted)
color	white