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HomeProduct name list5-Nitrofluorescein

5-Nitrofluorescein

5-Nitrofluorescein Structural

What is 5-Nitrofluorescein?

Chemical properties

Orange Cyrstalline Solid

The Uses of 5-Nitrofluorescein

Fluorescent labeling reagent for proteins, 6-Nitrofluorescein can be used in the fluorescent antibody technique for rapid identification of pathogens.

Preparation

5-Nitrofluorescein (3′,6′-dihydroxy-6-nitrospiro[2-benzofuran-3,9′-xanthene]-1-one) is a key intermediate in the synthesis of various fluorescent compounds such as 5-aminofluorescein (5-amino-3′,6′-dihydroxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one), fluorescein 5-isothiocyanate (FITC; 3′,6′-dihydroxy-5-isothiocyanato-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one), and fluorescein lisicol (previously known as cholyl-lysylfluorescein or CLF.
Process for the preparation of 5-/6-nitrofluorescein(US20120220787A1)

Properties of 5-Nitrofluorescein

solubility  Acetone, Methanol
form  Solid
color  Orange Cyrstalline
Stability: Light and Moisture Sensitive
InChI InChI=1S/C20H11NO7/c22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)20(14)16-7-10(21(25)26)1-4-13(16)19(24)28-20/h1-9,22-23H
EPA Substance Registry System Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-6-nitro- (27402-68-2)

Safety information for 5-Nitrofluorescein

Signal word Warning
Pictogram(s)
ghs
Exclamation Mark
Irritant
GHS07
GHS Hazard Statements H302:Acute toxicity,oral
H315:Skin corrosion/irritation
H319:Serious eye damage/eye irritation
H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes P261:Avoid breathing dust/fume/gas/mist/vapours/spray.
P280:Wear protective gloves/protective clothing/eye protection/face protection.
P301+P312:IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.
P302+P352:IF ON SKIN: wash with plenty of soap and water.
P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

Computed Descriptors for 5-Nitrofluorescein

InChIKey GYWGQAIDMYGOLU-UHFFFAOYSA-N
SMILES C12(C3=C(C=C(O)C=C3)OC3=C1C=CC(O)=C3)C1=C(C=CC([N+]([O-])=O)=C1)C(=O)O2

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