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HomeProduct name list5-BROMO-4-CHLORO-3-INDOLYL B-D-

5-BROMO-4-CHLORO-3-INDOLYL B-D-

Synonym(s):X-GlcA;X-glucuronide CHA salt

  • CAS NO.:114162-64-0
  • Empirical Formula: C20H26BrClN2O7
  • Molecular Weight: 521.79
  • MDL number: MFCD13185253
  • EINECS: 601-302-0
  • SAFETY DATA SHEET (SDS)
  • Update Date: 2024-10-28 16:48:35
5-BROMO-4-CHLORO-3-INDOLYL B-D- Structural

What is 5-BROMO-4-CHLORO-3-INDOLYL B-D-?

Chemical properties

White Solid

The Uses of 5-BROMO-4-CHLORO-3-INDOLYL B-D-

5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt has been used as a histochemical reagent in X-GLUC staining.

The Uses of 5-BROMO-4-CHLORO-3-INDOLYL B-D-

Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.
X-GlcA has been used in histochemical staining of root sections for light microscopic observation.

What are the applications of Application

XGLUC Cyclohexylammonium Salt is a chromogenic substrate for β-glucuronidase gene detection

General Description

X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Biochem/physiol Actions

5-bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt (X-GlcA) is a colorless substrate. The pre-immobilized X-GlcA can be converted by β-glucuronidase enzyme, synthesised by?Escherichia coli.

Properties of 5-BROMO-4-CHLORO-3-INDOLYL B-D-

Melting point: 228-230°C
alpha  -87~-94°(20℃/D)(c=1,H2O-DMF)(calculated on the dehydrous basis)
Density  1.4241 (rough estimate)
refractive index  1.6110 (estimate)
storage temp.  -20°C
solubility  DMF: soluble
form  tablet
color  White to off-white or colorless
Stability: Hygroscopic
EPA Substance Registry System .beta.-D-Glucopyranosiduronic acid, 5-bromo-4-chloro-1H-indol-3-yl, compd. with cyclohexanamine (1:1) (114162-64-0)

Safety information for 5-BROMO-4-CHLORO-3-INDOLYL B-D-

Signal word Warning
Pictogram(s)
ghs
Exclamation Mark
Irritant
GHS07
GHS Hazard Statements H302:Acute toxicity,oral
H315:Skin corrosion/irritation
H319:Serious eye damage/eye irritation
H332:Acute toxicity,inhalation
H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes P261:Avoid breathing dust/fume/gas/mist/vapours/spray.
P280:Wear protective gloves/protective clothing/eye protection/face protection.
P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

Computed Descriptors for 5-BROMO-4-CHLORO-3-INDOLYL B-D-

InChIKey PSBARFROSMJLJR-OXLZRBRISA-N

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