4-Methylumbelliferyl beta-D-galactoside

Synonym(s):MUG;MUGA

CAS NO.：6160-78-7
Empirical Formula: C16H18O8
Molecular Weight: 338.31
MDL number: MFCD00036773
EINECS: 228-185-5
SAFETY DATA SHEET (SDS)
Update Date: 2024-10-23 13:36:13

4-Methylumbelliferyl beta-D-galactoside Structural

What is 4-Methylumbelliferyl beta-D-galactoside?

Description

4-MUG (6160-78-7) is a versatile fluorogenic substrate for ?-galactosidase1 for use in cell extracts or purified enzyme preparations.1-3? This substrate produces a water soluble blue fluorescent coumarin fluorophore. Ex: 342 nm; Em: 441 nm

Chemical properties

White Powder

The Uses of 4-Methylumbelliferyl beta-D-galactoside

Substrate for fluorometric assay of ?-D-galactosidase

The Uses of 4-Methylumbelliferyl beta-D-galactoside

It is a substrate used for fluorometric assay for β-galactosidase. It is used to detect E-coli n the fluorogenic medium.

What are the applications of Application

4-Methylumbelliferyl β-D-galactopyranoside is a fluorescent substrate for b-galactosidase

Definition

ChEBI: A beta-D-galactoside having a 4-methylumbelliferyl substituent at the anomeric position.

References

Chiu et al. (2017), Measuring ?-Galactosidase Activity in Gram-Positive Bacteria Using a Whole-Cell Assay with MUG as a Fluorescent Reporter; Curr. Protoc. Toxicol., Supp 74 4.44.1 Kytidou et al. (2018), Nicotiana benthamiana α-galactosidase A1,1 can functionally complement human α-galactosidase A deficiency associated with Fabry disease; J. Biol. Chem., 293 10042 Hernandez-Guzman et al. (2016), Purification and characterization of an extracellular ?-glucosidase from Sporothrix schenckii; FEBS Open Bio., 6 1067

Properties of 4-Methylumbelliferyl beta-D-galactoside

Melting point:	258-263 °C (dec.)
Boiling point:	626.9±55.0 °C(Predicted)
alpha	-64 º (c=1, pyridine, after)
Density	1.522±0.06 g/cm3(Predicted)
storage temp.	-20°C
solubility	Soluble in DMSO (40 mg/ml)
form	Powder
pka	12.73±0.70(Predicted)
color	White to slightly off-white
Water Solubility	soluble
Sensitive	Moisture Sensitive & Hygroscopic
BRN	94673
Stability:	Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month.
CAS DataBase Reference	6160-78-7(CAS DataBase Reference)

Safety information for 4-Methylumbelliferyl beta-D-galactoside

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection.