2′,7′-Dichlorodihydrofluorescein Diacetate
Synonym(s):2ʹ,7ʹ-Dichlorofluorescin Diacetate - CAS 4091-99-0 - Calbiochem;H₂DCFDA, 2ʹ,7ʹ-Dichlorodihydrofluorescein Diacetate
- CAS NO.:4091-99-0
- Empirical Formula: C24H16Cl2O7
- Molecular Weight: 487.29
- MDL number: MFCD00128955
- EINECS: 685-843-8
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-11-07 21:39:59
What is 2′,7′-Dichlorodihydrofluorescein Diacetate?
Description
DCFH (commonly known as dichlorofluorescin) is used as an indicator of peroxynitrite formation. DCFH is supplied as the diacetate ester. Following enzymatic or base-
Chemical properties
white to yellow-beige or pale pink powder
The Uses of 2′,7′-Dichlorodihydrofluorescein Diacetate
Cell-permeable fluorogenic probe used to measure reactive oxygen species (ROS) within cells by detection of enzymatically formed hydrogen peroxide.
The Uses of 2′,7′-Dichlorodihydrofluorescein Diacetate
2',7'-Dichlorodihydrofluorescein as a cell-permeable non-fluorescent probe. It is de-esterified intracellularly and turns to highly fluorescent 2’7’dichlorofluorescein upon oxidation.
The Uses of 2′,7′-Dichlorodihydrofluorescein Diacetate
2′,7′-Dichlorofluorescin diacetate is a cell-permeable non-fluorescent probe. 2′,7′-Dichlorofluorescin diacetate is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation.
Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.
What are the applications of Application
2′,7′-Dichlorodihydrofluorescein Diacetate is a cell-permeable fluorogenic ROS probe
General Description
Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (?NO) and for the determination of the degree of overall oxidative stress.
Biochem/physiol Actions
Cell permeable: yes
storage
Store at -20°C
Properties of 2′,7′-Dichlorodihydrofluorescein Diacetate
Melting point: | 209-210 °C(lit.) |
Boiling point: | 593.5±50.0 °C(Predicted) |
Density | 1.463±0.06 g/cm3(Predicted) |
storage temp. | -20°C |
solubility | DMF: soluble |
form | Crystalline solid |
pka | 3.79±0.36(Predicted) |
color | White to off-white |
BRN | 4610273 |
Stability: | Light Sensitive |
EPA Substance Registry System | 2',7'-Dichlorohydrofluorescein diacetate (4091-99-0) |
Safety information for 2′,7′-Dichlorodihydrofluorescein Diacetate
Signal word | Warning |
Pictogram(s) |
Exclamation Mark Irritant GHS07 |
GHS Hazard Statements |
H302:Acute toxicity,oral H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation |
Precautionary Statement Codes |
P264:Wash hands thoroughly after handling. P264:Wash skin thouroughly after handling. P270:Do not eat, drink or smoke when using this product. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. P332+P313:IF SKIN irritation occurs: Get medical advice/attention. P337+P313:IF eye irritation persists: Get medical advice/attention. |
Computed Descriptors for 2′,7′-Dichlorodihydrofluorescein Diacetate
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