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HomeProduct name list2′,7′-Dichlorodihydrofluorescein Diacetate

2′,7′-Dichlorodihydrofluorescein Diacetate

Synonym(s):2ʹ,7ʹ-Dichlorofluorescin Diacetate - CAS 4091-99-0 - Calbiochem;H₂DCFDA, 2ʹ,7ʹ-Dichlorodihydrofluorescein Diacetate

  • CAS NO.:4091-99-0
  • Empirical Formula: C24H16Cl2O7
  • Molecular Weight: 487.29
  • MDL number: MFCD00128955
  • EINECS: 685-843-8
  • SAFETY DATA SHEET (SDS)
  • Update Date: 2024-11-07 21:39:59
2′,7′-Dichlorodihydrofluorescein Diacetate Structural

What is 2′,7′-Dichlorodihydrofluorescein Diacetate?

Description

DCFH (commonly known as dichlorofluorescin) is used as an indicator of peroxynitrite formation. DCFH is supplied as the diacetate ester. Following enzymatic or base-catalyzed cleavage of the diacetate groups, it is readily oxidized to the highly fluorescent product dichlorofluorescein (DHF). Peroxynitrite is an efficient mediator of this oxidation and neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DCFH. Formation of DHF can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 502 and 523 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 59,500 M−1cm−1).

Chemical properties

white to yellow-beige or pale pink powder

The Uses of 2′,7′-Dichlorodihydrofluorescein Diacetate

Cell-permeable fluorogenic probe used to measure reactive oxygen species (ROS) within cells by detection of enzymatically formed hydrogen peroxide.

The Uses of 2′,7′-Dichlorodihydrofluorescein Diacetate

2',7'-Dichlorodihydrofluorescein as a cell-permeable non-fluorescent probe. It is de-esterified intracellularly and turns to highly fluorescent 2’7’dichlorofluorescein upon oxidation.

The Uses of 2′,7′-Dichlorodihydrofluorescein Diacetate

2′,7′-Dichlorofluorescin diacetate is a cell-permeable non-fluorescent probe. 2′,7′-Dichlorofluorescin diacetate is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation.
Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.

What are the applications of Application

2′,7′-Dichlorodihydrofluorescein Diacetate is a cell-permeable fluorogenic ROS probe

General Description

Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (?NO) and for the determination of the degree of overall oxidative stress.

Biochem/physiol Actions

Cell permeable: yes

storage

Store at -20°C

Properties of 2′,7′-Dichlorodihydrofluorescein Diacetate

Melting point: 209-210 °C(lit.)
Boiling point: 593.5±50.0 °C(Predicted)
Density  1.463±0.06 g/cm3(Predicted)
storage temp.  -20°C
solubility  DMF: soluble
form  Crystalline solid
pka 3.79±0.36(Predicted)
color  White to off-white
BRN  4610273
Stability: Light Sensitive
EPA Substance Registry System 2',7'-Dichlorohydrofluorescein diacetate (4091-99-0)

Safety information for 2′,7′-Dichlorodihydrofluorescein Diacetate

Signal word Warning
Pictogram(s)
ghs
Exclamation Mark
Irritant
GHS07
GHS Hazard Statements H302:Acute toxicity,oral
H315:Skin corrosion/irritation
H319:Serious eye damage/eye irritation
Precautionary Statement Codes P264:Wash hands thoroughly after handling.
P264:Wash skin thouroughly after handling.
P270:Do not eat, drink or smoke when using this product.
P280:Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352:IF ON SKIN: wash with plenty of soap and water.
P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
P332+P313:IF SKIN irritation occurs: Get medical advice/attention.
P337+P313:IF eye irritation persists: Get medical advice/attention.

Computed Descriptors for 2′,7′-Dichlorodihydrofluorescein Diacetate

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