-N7-DGTP
Synonym(s):−N7-dGTP;7-Deaza-dGTP
- CAS NO.：101515-08-6
- Empirical Formula: C11H17N4O13P3
- Molecular Weight: 506.19
- MDL number: MFCD00171614
- SAFETY DATA SHEET (SDS)
- Update Date: 2023-05-04 15:16:32
What is -N7-DGTP?
The Uses of -N7-DGTP
7-Deaza-dGTP is a substrate for most DNA polymerases, including Taq DNA polymerase.
7-Deaza-dGTP is used in the dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA.
Partial substitution of 7-Deaza-dGTP for dGTP in PCRs can improve the product yield when the template is GC-rich (i.e., has extensive secondary structures).
General Description
7-Deaza-2′-deoxyguanosine 5′-triphosphate enhances polymerase chain reaction (PCR) product yield.
Biochem/physiol Actions
This dGTP analog pairs weakly with conventional bases to minimize DNA secondary structures, while being a good substrate for DNA ploymerases.
Properties of -N7-DGTP
| Density | 2.48±0.1 g/cm3(Predicted) |
| storage temp. | -20°C |
| pka | 0.97±0.50(Predicted) |
| form | solution |
| color | colorless |
Safety information for -N7-DGTP
Computed Descriptors for -N7-DGTP
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