IPTG
Synonym(s):IPTG;Isopropyl-β-D-thiogalactopyranoside;IPTG solution;Isopropyl β-D -1-thiogalactopyranoside;Isopropyl β-D -thiogalactoside
- CAS NO.:367-93-1
- Empirical Formula: C9H18O5S
- Molecular Weight: 238.3
- MDL number: MFCD00063273
- EINECS: 206-703-0
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-06-04 15:29:52
What is IPTG?
Description
IPTG (also known as Isopropyl-β-D-Thiogalactopyranoside) is a molecular biology reagent that functions as an inducer of galactosidase activity by binding to and inhibiting the repressor. It is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator.
IPTG cannot be hydrolyzed or broken down by the E. coli therefore the concentration remains constant during cell replication. IPTG is often used at a final concentration ranging from 0.5-1.0 mM. X-Gal is often used with IPTG since it provides an easy visual method to monitor whether protein expression has occurred. X-Gal as the name implies contains a galactose group that once metabolized causes a bright blue color change.
The Uses of IPTG
Isopropyl-β-D-thiogalactoside is used as a reagent in molecular biology. It is used as an effective β-galactosidase inducer of protein expression where the gene is under the control of the lac operator. It is used with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside to identify the non-recombinant plasmid in cloning experiments by blue-white screen methodology.
Pharmacokinetics
IPTG (Isopropyl ?-D-1-thiogalactopyranoside), is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon and it is therefore used to induce protein expression where the gene is under the control of the lac operator.
Properties of IPTG
Melting point: | 105 °C |
Boiling point: | 350.9°C (rough estimate) |
Density | 1.3329 (rough estimate) |
storage temp. | 2-8°C |
solubility | Soluble in Water or Methanol. |
form | Crystalline Powder |
color | White |
Water Solubility | soluble |
Safety information for IPTG
Signal word | Warning |
Pictogram(s) |
Exclamation Mark Irritant GHS07 Health Hazard GHS08 |
GHS Hazard Statements |
H319:Serious eye damage/eye irritation H351:Carcinogenicity |
Precautionary Statement Codes |
P281:Use personal protective equipment as required. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. |
Computed Descriptors for IPTG
InChIKey | BPHPUYQFMNQIOC-NXRLNHOXSA-N |
Abamectin manufacturer
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Neugen Labs
Rivashaa Agrotech Biopharma Pvt. Ltd.
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