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HomeProduct name listThrombin

Thrombin

Synonym(s):Factor IIa;Thrombin, Plasminogen-Free, Bovine

  • CAS NO.:9002-04-4
  • Empirical Formula: NULL
  • Molecular Weight: 0
  • MDL number: MFCD00166053
  • EINECS: 232-648-7
  • SAFETY DATA SHEET (SDS)
  • Update Date: 2024-10-23 13:36:13

What is Thrombin?

Description

In order to stop local bleeding, thrombin, a natural thrombin drug that catalyzes the transformation of fibrinogen to fibrin, is frequently used. Thrombin is synthesized from cow plasma, and it is used to stop bleeding from open vessels when it is not possible to use other methods. It is used to stop light bleeding.

The Uses of Thrombin

Thrombin is a trypsin-like serine protease involved in a multitude of processes in the human body. Thrombin generation is the result of limited proteolysis of the vitamin K-dependent zymogen prothrombin. Thrombin is the last enzyme in the clotting cascade functioning to cleave fibrinogen to fibrin which forms the fibrin gel of a haemostatic plug or a pathologic thrombus

The Uses of Thrombin

Thrombostat (Parke-Davis).

The Uses of Thrombin

Thrombin is used in coagulation research, medical research, protein-structure analysis, and biochemical research. It has been used for the stimulation of the platelets.

General Description

Thrombin is a sterile protein substanceprepared from prothrombin of bovine origin. It is used as atopical hemostatic because it can clot blood, plasma, or a solutionof fibrinogen without addition of other substances.Thrombin also may initiate clotting when combined withgelatin sponge or fibrin foam.
For external use it is applied topically to the wound, as asolution containing 100 to 2,000 National Institutes ofHealth (NIH) units/mL in sodium chloride irrigation or sterilewater for injection or as a dry powder.

Biochem/physiol Actions

The main function of thrombin is the cleavage of fibrinogen to fibrin, to assist stable clot formation. A wide range of mutations in the prothrombin gene contributes to its deficiency resulting in coagulation disorders like dysprothrombinemia and hypoprothrombinemia. High levels of thrombin elicit neurotoxicity in dopaminergic neurons and contributes to the progression of Parkinson′s disease. Altered thrombin levels modulates the coagulation pathway in multiple sclerosis. Patients with coronary artery disease (CAD) show elevated levels of thrombin. Thrombin accumulation in neurofibrillary tangles of the brain may contribute to the aggregation of tau protein and pathophysiology of Alzheimer disease.

Purification Methods

Thrombin is purified by chromatography on a DEAE-cellulose column, while eluting with 0.1M NaCl, pH 7.0, followed by chromatography on Sephadex G-200. The final preparation is free from plasminogen and plasmin. [Yin & Wessler J Biol Chem 243 112 1968.] Thrombin from bovine blood is also purified by chromatography using p-chlorobenzylamino--aminocaproyl agarose, and gel filtration through Sephadex G-25. [Thompson & Davie Biochim Biophys Acta 250 210 1971.] Thrombin from various species was purified initially by precipitation of impurities with rivanol. [Miller Nature 184 450 1959.] Tissue inhibitor of metalloproteins (TIMP, from human blood plasma), Mr ~30,000. These are purified by an [anti-human amniotic fluid-TIMP]-Sepharose immuno-affinity column and eluted with 50mM glycine/HCl pH 3.0 buffer that is 0.5M in NaCl, and followed by gel filtration [Cawston et al. Biochem J 238 677 1986].

Properties of Thrombin

Melting point: 47.7 °C
storage temp.  -20°C
solubility  H2O: 1 mL/vial clear to slightly hazy, colorless solution
form  powder
color  white
Merck  13,9461
EPA Substance Registry System Thrombin (9002-04-4)

Safety information for Thrombin

Signal word Danger
Pictogram(s)
ghs
Exclamation Mark
Irritant
GHS07
ghs
Health Hazard
GHS08
GHS Hazard Statements H315:Skin corrosion/irritation
H319:Serious eye damage/eye irritation
H334:Sensitisation, respiratory
H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes P261:Avoid breathing dust/fume/gas/mist/vapours/spray.
P264:Wash hands thoroughly after handling.
P264:Wash skin thouroughly after handling.
P271:Use only outdoors or in a well-ventilated area.
P302+P352:IF ON SKIN: wash with plenty of soap and water.
P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

Computed Descriptors for Thrombin

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