Rhodamine 123

Description

Rhodamine 123 is a membrane-permeable cationic dye that is readily accumulated within living cells. It is a substrate for the efflux pump P-glycoprotein (P-gp; also known as multidrug resistance protein 1 and ABCB1) and is rapidly exported from cells with functional P-gp. As P-gp is expressed on a population of stem cells known as the side population, rhodamine 123 is used to detect this group of stem cells. Rhodamine 123 also accumulates within mitochondria due to its positive charge and can inhibit oxidative phosphorylation. Rhodamine 123 has excitation/emission maxima of 507/529 nm.

Chemical properties

red-brown to brown or dark green powder

The Uses of Rhodamine 123

Rhodamine-123 is a fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Rhodamine 123 (R123), as a typical of P-glycoprotein substrate, was widely used to quantify P-glycoprotein (P-gp) functional efflux activity in vivo. Rhodamine-123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid).

The Uses of Rhodamine 123

Rhodamine derivative, a fluoresent dye-labeled compound.

The Uses of Rhodamine 123

Fluorescent dye most commonly used as functional reporter in flow cytometry.

The Uses of Rhodamine 123

Rhodamine-123 lycoprotein (P-gp) functional efflux activity in vivo. Rhodamine-123 PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid).

The Uses of Rhodamine 123

Useful as a laser dye and to have selective cell growth effects. Mitochondrial specific fluorescent dye and substrate for P-glycoprotein.

What are the applications of Application

Rhodamine 123 is a cationic dye that emits a green fluorescence

in vitro

rhodamine 123, which was identified as a member of the rhodamine family of flurone dyes, was used to examine membrane transport by the abcb1 gene product, mdr1. previous study determined the λmax for excitation and emission for rhodamine 123 in commonly used solvents and extraction buffers, indicating that the fluorescence of rhodamine 123 was highly dependent on the chemical environment. the optimal parameters are 1% methanol in hbss. in addition, the uptake of rhodamine 123 into cells was via both passive and active processes, and this occurred mainly by oatp1a2-mediated transport. furthermore, this previous study quantified the intracellular sequestration and metabolism of rhodamine 123, showing that these were both cell line-dependent factors that might influence the interpretation of transport assays [1].

References

[1] samantha forster et al. characterization of rhodamine-123 as a tracer dye for use in in vitro drug transport assays. plos one. 2012; 7(3): e33253.