RESORUFIN SODIUM SALT
Synonym(s):7-Hydroxy-3H-phenoxazin-3-one;7-Hydroxy-3H-phenoxazin-3-one sodium salt;NSC 12097
- CAS NO.:34994-50-8
- Empirical Formula: C12H6NNaO3
- Molecular Weight: 235.17
- MDL number: MFCD00005037
- EINECS: 252-310-2
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-10-31 20:01:59
What is RESORUFIN SODIUM SALT?
Chemical properties
Dark Purple Solid
The Uses of RESORUFIN SODIUM SALT
Resorufin is a pink fluorescent dye that is not fluorescent until activated by UV light. Resorufin is an anionic tricyclic dye.
The Uses of RESORUFIN SODIUM SALT
Resorufin has been used to determine the cytochrome P450 (CYP) 2A5 coumarin 7-hydroxylase (COH) activity changes over a broad range of lipopolysaccharide (LPS) doses. It has also been used as an internal standard for ethoxyresurufin-o-deethylase (EROD) analysis.
What are the applications of Application
Resorufin sodium salt is an anionic tricyclic phenoxazineone dye compound
General Description
Resorufin is a fluorescent indicator.
Purification Methods
The free acid is purified by dissolving it in conc H2SO4 which on dilution precipitates resorufin and is filtered off, washed well with hot H2O and dried. [Eichler J Prakt Chem 139 113 1934.] Detailed UV and IR studies were reported by Musso & Mattheis Chem Ber 90 1814 1957]. [pKa: Musso & Rathjen Chem Ber 92 751 1959, Beilstein 27 1V 2263.] The monoacetate [1152-14-3] M 252.2, m 2 2 3o is a fluorogenic substrate used for hydrolytic enzymes, and its fluorescence has Emax 593nm (Eex 500nm, in 0.1M phosphate pH 8.0 with lipase) [Kitson & Kitson Biochem J 322 701 1997]. [Beilstein 27 IV 2263.] The O7-methylresorufin (7-methoxy-3H-phenoxazin-3-one) [5735-89-3] M 227.2, m > 2 2 0o has fluorescence at Emax 585nm (Eexcit 571 nm in deacylase solution) and is used to differentiate isoenzymes of cytochrome P-450. It is insoluble in H2O and dilute alkali but is soluble in EtOH and CHCl3 to give an orange yellow colour. [Kehrmann Justus Liebigs Ann Chem 372 352 1910, Beilstein 27 II 108.]
Properties of RESORUFIN SODIUM SALT
Melting point: | >300 °C(lit.) |
storage temp. | room temp |
solubility | Methanol (Slightly), Water (Slightly) |
form | Solid |
color | Dark Purple to Black |
BRN | 174850 |
Safety information for RESORUFIN SODIUM SALT
Signal word | Warning |
Pictogram(s) |
Exclamation Mark Irritant GHS07 |
GHS Hazard Statements |
H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation |
Precautionary Statement Codes |
P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P264:Wash hands thoroughly after handling. P264:Wash skin thouroughly after handling. P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. |
Computed Descriptors for RESORUFIN SODIUM SALT
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