P-nitrophenyl butyrate
Synonym(s):p-Nitrophenyl butyrate;Butyric acid 4-nitrophenyl ester
- CAS NO.:2635-84-9
- Empirical Formula: C10H11NO4
- Molecular Weight: 209.2
- MDL number: MFCD00047734
- EINECS: 258-258-3
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-07-02 08:54:57
What is P-nitrophenyl butyrate?
Description
P-nitrophenyl butyrate also kown as 4-nitrophenyl butyrate, is a probe for lipase activity. It is hydrolyzed by lipase in the water ambient condition, giving rise to p-nitrophenol and butyric acid. P-nitrophenol actually occurs in the anionic form, p-nitrophenolate, giving a yellowish coloration and absorbing light at 410 nm in an alkaline buffer such as Tris-HCl pH 7.5.
The Uses of P-nitrophenyl butyrate
A probe for lipase activity.
The Uses of P-nitrophenyl butyrate
4-Nitrophenyl butyrate is a substrate for esterase, and lipase enzyme. Hydrolysis of 4-nitrophenyl butyrate by these enzymes releases the chromophore, 4-nitrophenolate which is spectrophotometrically analysed at 415 nm.
What are the applications of Application
4-Nitrophenyl butyrate is a probe for lipase activity
Definition
ChEBI: P-nitrophenyl butyrate is a butyrate ester resulting from the formal condensation of the hydroxy group of 4-nitrophenol with the carboxy group of butyric acid. It is a butyrate ester and a C-nitro compound. It is functionally related to a 4-nitrophenol.
References
[1] POHANKA M. Biosensors and Bioassays Based on Lipases, Principles and Applications, a Review.[J]. Molecules, 2019. DOI:10.3390/molecules24030616.
[2] WENTING SHANG. Enzymatic hydrolysis of p-nitrophenyl butyrate in CO2-based micelle systems[J]. Journal of chemical technology and biotechnology, 2013, 89 9: 1324-1329. DOI:10.1002/jctb.4246.
[3] SIGRUN H. STERRI Frode F Bj?rn A Johnsen. A radiochemical assay method for carboxylesterase, and comparison of enzyme activity towards the substrates methyl [1-14C] butyrate and 4-nitrophenyl butyrate[J]. Biochemical pharmacology, 1985, 34 15: Pages 2779-2785. DOI:10.1016/0006-2952(85)90579-9.
[4] BISHNU PRASAD PANDEY. Bergenia pacumbis from Nepal, an astonishing enzymes inhibitor.[J]. BMC Complementary Medicine and Therapies, 2020: 198. DOI:10.1186/s12906-020-02989-2.
Properties of P-nitrophenyl butyrate
Boiling point: | 184-185 °C |
Density | 1.19 g/mL at 20 °C(lit.) |
storage temp. | -20°C |
solubility | chloroform: 0.1 g/mL, clear, faintly yellow |
form | A neat oil |
color | Colorless to light yellow |
BRN | 1879729 |
Safety information for P-nitrophenyl butyrate
Signal word | Warning |
Pictogram(s) |
Exclamation Mark Irritant GHS07 |
GHS Hazard Statements |
H317:Sensitisation, Skin |
Precautionary Statement Codes |
P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P272:Contaminated work clothing should not be allowed out of the workplace. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of soap and water. P333+P313:IF SKIN irritation or rash occurs: Get medical advice/attention. |
Computed Descriptors for P-nitrophenyl butyrate
InChIKey | DVDUMIQZEUTAGK-UHFFFAOYSA-N |
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