Nucleoside phosphorylase

Synonym(s):Nucleoside Phosphorylase bacterial;PNP;Purine nucleoside phosphorylase;Purine nucleoside:orthophosphate ribosyltransferase

CAS NO.：9030-21-1
Empirical Formula: C10H12N4O4
Molecular Weight: 252.22668
MDL number: MFCD00131739
EINECS: 232-857-3
SAFETY DATA SHEET (SDS)
Update Date: 2024-07-02 08:55:37

What is Nucleoside phosphorylase?

Chemical properties

Powder

The Uses of Nucleoside phosphorylase

Nucleoside Phosphorylase from microorganisms has been used:

in phosphopentomutase assay for adenosine synthesis
for the conversion of 7-methylthioguanosine to 7-methylthioguanine
in the synthesis of cytokinins in lyophilized cotton plant samples

General Description

Nucleoside Phosphorylase are trimeric and have a molecular mass of about 100 kDa. Each monomer consists of a mixed β-sheet core flanked by eight α-helices and a purine binding in hydrophobic pocket.

Biochem/physiol Actions

Purine nucleoside phosphorylase is an enzyme involved in purine metabolism. PNP metabolizes adenosine into adenine, inosine into hypoxanthine, and guanosine into guanine. Mutations in the PNP gene are responsible for purine nucleoside phosphorylase deficiency.

Properties of Nucleoside phosphorylase

storage temp.	-20°C
form	lyophilized powder
color	White to off-white
InChI	InChI=1/C10H12N4O4/c15-2-6-7(16)8(17)10(18-6)14-4-13-5-1-11-3-12-9(5)14/h1,3-4,6-8,10,15-17H,2H2/t6-,7-,8-,10-/s3