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HomeProduct name listMethyl Green

Methyl Green

Synonym(s):Ethyl Green

  • CAS NO.:82-94-0
  • Empirical Formula: C26H33ClN3+
  • Molecular Weight: 423.02
  • MDL number: MFCD00011879
  • EINECS: 201-447-6
  • SAFETY DATA SHEET (SDS)
  • Update Date: 2023-06-08 17:06:34
Methyl Green Structural

What is Methyl Green?

Description

Methyl Green is a DNA stain used as a tracking dye in acid buffers for electrophoresis. It helps differentiate bacteria and is also a tissue stain for nucleoli and mitochondria in tissues. It is used with Bismarck Brown frequently.

The Uses of Methyl Green

Methyl Green powder dye is used in various staining methods in microscopy. It is also used with Pyronine Y dye for one stage DNA (green) and RNA (red) staining method. It is also used as a counterstain with other reactions, such as demonstration of enzymatic activity and for mitochondrial staining according to Altmann. Methyl green solution is a suitable counterstain for chloroacetate esterase, nonspecific esterase, alkaline phosphatase, peroxidase, naphthol AS acetate esterase, and acid phosphatase.

Definition

ChEBI: Methylene green is an organic chloride salt having 3,7-bis(dimethylamino)-4-nitrophenothiazin-5-ium as the counterion. It stains nuclei green and is sometimes used as a counterstain to red or purple primary stains. It has a role as a fluorochrome and a histological dye. It contains a 3,7-bis(dimethylamino)-4-nitrophenothiazin-5-ium.

Preparation

chlorine methane and (C.I.Basic Violet 1, C.I. 42535) reaction

Purification Methods

Crystallise the dye from hot water. [Beilstein 13 IV 2286.]

Properties and Applications

green light blue. Soluble in water for blue light green, not soluble in ethanol and e alcohol. The strong sulfuric acid to red light yellow, yellow green for diluted. Dyes to join sodium hydroxide solution to colorless.

Standard( Cotton ) Light Fastness Persperation Fastness Ironing Fastness Soaping
Fading Stain Fading Stain Fading Stain
C 1 2

Properties of Methyl Green

Water Solubility  Soluble in water
solubility  Methanol (Slightly), Water (Slightly)
form  Liquid
Colour Index  42585
color  Clear blue-green
PH Range 0.1(yellow)-2.3(blue)
Stability: Hygroscopic
CAS DataBase Reference 82-94-0
EPA Substance Registry System C.I. Basic Blue 20 (82-94-0)

Safety information for Methyl Green

Computed Descriptors for Methyl Green

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