L-GLUTAMIC ACID GAMMA-(P-NITROANILIDE) HYDROCHLORIDE

Synonym(s):L-γ-Glutamyl-p-nitroanilide

CAS NO.：67953-08-6
Empirical Formula: C11H14ClN3O5
Molecular Weight: 303.7
MDL number: MFCD00039054
EINECS: 267-944-5
SAFETY DATA SHEET (SDS)
Update Date: 2024-11-01 14:32:37

L-GLUTAMIC ACID GAMMA-(P-NITROANILIDE) HYDROCHLORIDE Structural

What is L-GLUTAMIC ACID GAMMA-(P-NITROANILIDE) HYDROCHLORIDE?

Description

L-γ-Glutamyl-p-nitroanilide (67953-08-6; GPNA) is a substrate for γ-glutamyl transpeptidase. GPNA is used for the determination of γ-glutamyl transpeptidase hydrolytic activity via spectrophotometric monitoring of p-nitroaniline released by the action of the enzyme on GPNA.1 GPNA has also been shown to be an inhibitor of glutamine uptake – solute-linked carrier family A1 member 5 (SLC1A5).2?Cellular uptake of L-glutamine (regulated by SLC1A5) is a rate-limiting step that activates mTOR and is, thus, an interesting cancer chemotherapy target.3

The Uses of L-GLUTAMIC ACID GAMMA-(P-NITROANILIDE) HYDROCHLORIDE

L-Glutamic acid gamma-(p-nitroanilide) is used in methods of treating cancers having a deregulated NRF2/KEAP1 pathway.

What are the applications of Application

L-Glutamic acid γ-(p-nitroanilide) hydrochloride is a substrate for gamma-glutamyl transpeptidase (GGT).

References

1) Meister?et al. (1981),?γ-Glutamyl transpeptidase; Methods Enzymol.,?77?237 2) Esslinger?et al. (2005),?N-gamma-aryl glutamine analogues as probes of the ASCT2 neutral amino acid transporter binding site; Bioorg. Med. Chem.,?13?1111 3) Nicklin?et al. (2012),?Bidirectional Transport of Amino Acid Regulates mTOR and Autophagy; Cell,?136?521

Properties of L-GLUTAMIC ACID GAMMA-(P-NITROANILIDE) HYDROCHLORIDE

storage temp.	2-8°C
solubility	Soluble in Water (up to 5 mg/ml).
form	solid
color	Off-white
Stability:	Stable for 1 year from date of purchase as supplied. Solutions in distilled water may be stored at -20° for up to 1 month.
EPA Substance Registry System	L-Glutamine, N-(4-nitrophenyl)-, monohydrochloride (67953-08-6)

Safety information for L-GLUTAMIC ACID GAMMA-(P-NITROANILIDE) HYDROCHLORIDE

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H302:Acute toxicity,oral H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.