HEPES sodium salt

Synonym(s):4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt;N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt;N-2-Hydroxyethylpiperazine-Nʹ-2-ethanesulfonic acid, Sodium Salt

CAS NO.：75277-39-3
Empirical Formula: C8H19N2NaO4S
Molecular Weight: 262.3
MDL number: MFCD00036463
EINECS: 278-169-7
SAFETY DATA SHEET (SDS)
Update Date: 2024-12-18 14:15:30

What is HEPES sodium salt?

Chemical properties

white powder

The Uses of HEPES sodium salt

HEPES sodium salt solution is suitable for cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during the phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

What are the applications of Application

HEPES Sodium salt is a zwitterionic all-purpose buffer

What are the applications of Application

HEPES sodium salt solution (1M) is an all-purpose buffer for use in biological research. 0.2 μm filtered

Definition

ChEBI: Sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate is an organic sodium salt, a N-(2-hydroxyethyl)piperazine and a N-(sulfoalkyl)piperazine. It contains a 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate.

General Description

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of 6.8 to 8.2.

Flammability and Explosibility

Not classified

Biological Activity

Multi purpose buffer used in biological research.

storage

Room temperature

Properties of HEPES sodium salt

Melting point:	234 °C
Density	1.504[at 20℃]
vapor pressure	0Pa at 25℃
storage temp.	room temp
solubility	H₂O: 1 M at 20 °C, clear, colorless
form	dry powder
color	White
PH	10.0-12.0 (1M in H2O)
pka	7.5(at 25℃)
PH Range	6.8 - 8.2
Water Solubility	It is soluble in water.
Sensitive	Hygroscopic
Stability:	Stable. Hygroscopic - protect from moisture. Incompatible with strong oxidizing agents.
CAS DataBase Reference	75277-39-3(CAS DataBase Reference)
EPA Substance Registry System	1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, monosodium salt (75277-39-3)

Safety information for HEPES sodium salt

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P280:Wear protective gloves/protective clothing/eye protection/face protection. P304+P340:IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.