DEOXYRIBONUCLEASE II
Synonym(s):Deoxyribonucleate 3′-oligonucleotido-hydrolase;DNase II
- CAS NO.：9025-64-3
- Empirical Formula: NULL
- Molecular Weight: 0
- MDL number: MFCD00130919
- EINECS: 232-801-8
- Update Date: 2024-07-02 08:55:14
What is DEOXYRIBONUCLEASE II?
The Uses of DEOXYRIBONUCLEASE II
Deoxyribonuclease II from bovine spleen has been used:
- as a component of digestion mix to isolate immune cells from colon samples
- to digest retinal explants for single-cell suspension preparation
- for isolation of stromal cells from human colon tissue
- in the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart
The Uses of DEOXYRIBONUCLEASE II
Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.
Biochem/physiol Actions
Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3′-phosphates. in vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.
Properties of DEOXYRIBONUCLEASE II
| storage temp. | -20°C |
| form | lyophilized powder |
| color | White to light yellow |
| EPA Substance Registry System | Nuclease, deoxyribo-, II (9025-64-3) |
Safety information for DEOXYRIBONUCLEASE II
Computed Descriptors for DEOXYRIBONUCLEASE II
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