DANSYL ETHYLENEDIAMINE

Synonym(s):N-(2-Aminoethyl)-5-dimethylamino-1-naphthalenesulfonamide;N-(2-Aminoethyl)dansylamide;DNSEDA;NSC 666752

CAS NO.：35060-08-3
Empirical Formula: C14H19N3O2S
Molecular Weight: 293.38
MDL number: MFCD00467523
EINECS: 233-305-4
SAFETY DATA SHEET (SDS)
Update Date: 2024-08-11 15:28:25

What is DANSYL ETHYLENEDIAMINE?

Description

Dansyl ethylenediamine is a lipophilic fluorophore that can be coupled to coarboxylic acids of proteins and other water-soluble polymers. The primary aliphatic amine of dansyl ethylenediamine can be reversibly coupled to aldehydes and ketones to form a Schiff base, which can be reduced to a generate stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNH3). Spectral data: λex=335nm, λem=525nm in methanol. Dansyl ethylenediamine has also shown to inhibit human renal dipeptidase (hrDP) irreversibly in a time-dependent manner.

Chemical properties

Yellow to orange powder.

The Uses of DANSYL ETHYLENEDIAMINE

A fluorescent reagent

The Uses of DANSYL ETHYLENEDIAMINE

A fluorescent reagent.

References

[1] YUKI INOUE. Fluorescent molecularly imprinted polymer thin films for specific protein detection prepared with dansyl ethylenediamine-conjugated O-acryloyl l-hydroxyproline[J]. Biosensors and Bioelectronics, 2013. DOI:10.1016/j.bios.2013.03.005.
[2] ADACHIH. Importance of Glu-125 in the catalytic activity of human renal dipeptidase.[J]. Biochimica et biophysica acta, 1993. DOI:10.1016/0167-4838(93)90276-w.
[3] MALLAMPATI S, AERSCHOT A V, HOOGMARTENS J, et al. Analysis of dideoxyadenosine triphosphate by CE with fluorescence detection. I. Derivatization through the phosphate group[J]. ELECTROPHORESIS, 2007, 28. DOI:10.1002/elps.200700234.
[4] DUDLEY R W, SLOTTERBECK B D, MCINERNEY M F, et al. Fatty acylation enhances cellular localization of dansylated phenylalanines[J]. Molecular and Cellular Biochemistry, 2010, 346: 81-87. DOI:10.1007/s11010-010-0593-4.

Properties of DANSYL ETHYLENEDIAMINE

Melting point:	145-146°C
Boiling point:	470.2±55.0 °C(Predicted)
Density	1.262±0.06 g/cm3(Predicted)
storage temp.	-20°C Freezer
solubility	methanol: soluble1mg/mL, clear, slightly yellow to deep yellow
form	Solid
pka	11.30±0.50(Predicted)
color	Light Yellow
BRN	2817535

Safety information for DANSYL ETHYLENEDIAMINE

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H302:Acute toxicity,oral H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.