CARBOXYPEPTIDASE B
Synonym(s):Peptidyl-L -lysine(L -arginine) hydrolase;Protaminase
- CAS NO.:9025-24-5
- Empirical Formula: C31H38N4O7S
- Molecular Weight: 610.72102
- MDL number: MFCD00081476
- EINECS: 232-788-9
- SAFETY DATA SHEET (SDS)
- Update Date: 2023-10-26 18:06:12
What is CARBOXYPEPTIDASE B?
The Uses of CARBOXYPEPTIDASE B
The enzyme from Sigma has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format. It has been used to evaluate the impact of the C-terminal lysine(s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis.
The Uses of CARBOXYPEPTIDASE B
Carboxypeptidase B from Sigma has been used as a reference for assaying carboxypeptidase activity in lysed pituitary granules derived from the anterior and intermediate lobes of rat. The enzyme has also been used to digest plasma samples by removing C-terminal basic amino acids, to get a distinct band for each allotype during C4 electrophoresis.
General Description
Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides. Inhibited by EDTA and other Zn2+ chelators.
Biochem/physiol Actions
Carboxypeptidase B is a proteolytic enzyme capable of rapidly hydrolyzing peptide bonds to release certain carboxyl-terminal basic amino acids from peptides and proteins. Its molecular mass is 34,300±600 Da. It contains one non-dialyzable gram atom of zinc per mole. The enzyme activity is inhibited by metal chelating agents 1, 10-phenanthroline, 8-hydroxyquinoline-5-sulfonic acid, and 2,2′-dipyridyl.
Properties of CARBOXYPEPTIDASE B
storage temp. | -20°C |
form | powder |
color | white |
Safety information for CARBOXYPEPTIDASE B
Signal word | Danger |
Pictogram(s) |
Exclamation Mark Irritant GHS07 Health Hazard GHS08 |
GHS Hazard Statements |
H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H334:Sensitisation, respiratory H335:Specific target organ toxicity, single exposure;Respiratory tract irritation |
Precautionary Statement Codes |
P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. |
Computed Descriptors for CARBOXYPEPTIDASE B
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ZIRCONIUM AAS SOLUTION 99%View Details
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FUCHSIN BASIC 0.1% SOLUTION 99%View Details
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1 10-PHENANTHROLIN HYDROCHLORIDE 99%View Details
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OXALIC ACID SOLUTION 0.1 N 99%View Details
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A-AMYLASE (DIASTASE) 99%View Details