Calpain Inhibitor I
Synonym(s):ALLN - CAS 110044-82-1 - Calbiochem;Calpain Inhibitor I, LLNL, MG 101, Proteasome Inhibitor V;InSolution ALLN - CAS 110044-82-1 - Calbiochem;LLNL, MG 101, Calpain Inhibitor I, Proteasome Inhibitor V
- CAS NO.:110044-82-1
- Empirical Formula: C20H37N3O4
- Molecular Weight: 383.53
- MDL number: MFCD00065505
- EINECS: 999-999-2
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-10-30 18:52:02
What is Calpain Inhibitor I?
Description
ALLN (110044-82-1) is a cell-permeable, peptide aldehyde inhibitor of calpain I (Ki=190 nM), calpain II (Ki=150 nM), cathepsin L (Ki=0.5 nM) and other neutral cysteine proteases.1?Inhibits cell cycle progression at G1/S?and metaphase/anaphase in CHO cells by inhibiting cyclin B degradation.2?Inhibits proteolytic degradation of IκBα and IκBβ in RAW macrophages induced with LPS.3?Blocks AIF-mediated necroptosis.4?Cell permeable.
Chemical properties
white to off-white powder
The Uses of Calpain Inhibitor I
Calpain inhibitor I reversibly blocks the calcium-dependent neutral cysteine protease calpain I. Calpains have been implicated in various cellular processes, including cell proliferation, apoptosis, and cell differentiation.
The Uses of Calpain Inhibitor I
It inhibits apoptosis in thymocytes and metamyelocytes. It also inhibits cell cycle progression at the G1/S border and metaphase/anaphase in CHO cells by inhibiting cyclin B degradation. ALLN inhibits the proteolysis of IκBα and IκBβ by the ubiquitin-proteasome complex. ALLN also prevents nitric oxide production by activated macrophages by interfering with transcription of the inducible nitric oxide synthase gene.
The Uses of Calpain Inhibitor I
Calpain inhibitor I inhibits calpain, which activates myosin light chain kinase and protein kinase C by partial proteolysis. The inhibitor should be membrane permeable due to low molecular weight and lack of charged residues.
Calpain Inhibitor I is used for western blotting methods.
What are the applications of Application
ALLN is a peptide aldehyde inhibitor of calpains and cathepsins
What are the applications of Application
Calpain Inhibitor I is a calpain and cathepsin inhibitor
Definition
ChEBI: A tripeptide composed of N-acetylleucyl, leucyl and norleucinal residues joined in sequence.
General Description
Calpain Inhibitor I is a strong, competitive inhibitor of the Ca2+-dependent neutral cysteine proteases calpain I and calpain II.
Biological Activity
Calpain inhibitor (IC 50 = 0.09 μ M) that activates p53-dependent apoptosis in tumor cell lines. Increases activated p53, p21 and caspase levels and promotes cell cycle arrest through inhibition of cyclin D degradation in vitro . Also attenuates ischemia/reperfusion injury in cardiomyocytes, hepatocytes and renal tubular cells through downregulation of iNOS and COX-2 expression.
Biochem/physiol Actions
Calpain Inhibitor I inhibits tumor necrosis factor-induced (TNF-induced) cleavage of Poly (ADP-ribose) polymerase (PARP) and calpastatin in apoptosis. It also inhibits oxygen/glucose deprivation (OGD) induced cleavage of caspase-12 and Bcl-xL..
References
1) Sasaki?et al.?(1990),?Inhibitory effect of di- and tripeptidyl aldehydes on calpains and cathepsins; J. Enzyme Inhib.?3?195 2) Sherwood?et al. (1993)?In vivo inhibition of cyclin B degradation and induction of cell-cycle arrest in mammalian cells by the neutral cysteine protease inhibitor N-acetylleucylleucylnorleucinal; Proc. Natl. Acad. Sci USA?90?3353 3) Schow and Joly (1997),?N-acetyl-leucinyl-leucinyl-norleucinal inhibits lipopolysaccharide-induced NF-kappaB activation and prevents TNF and IL-6 synthesis in vivo; Cell Immunol.?175?199 4) Shang?et al.?(2014)?Calpain: a molecule to induce AIF-mediated necroptosis in RGC-5 following elevated hydrostatic pressure; BMC Neurosci.?15?63
Properties of Calpain Inhibitor I
Melting point: | 182 °C |
Boiling point: | 510.42°C (rough estimate) |
Density | 1.1322 (rough estimate) |
refractive index | 1.6300 (estimate) |
storage temp. | 2-8°C |
solubility | DMSO: soluble |
pka | 13.53±0.46(Predicted) |
form | powder |
color | white |
Water Solubility | Soluble in dimethyl sulfoxide, ethanol, methanol. Insoluble in water, and aqueous buffers. |
BRN | 7656053 |
Stability: | Stable for 1 year from date of purchase as supplied. Solutions in DMSO or ethanol may be stored at -20°C for up to 1 month. |
Safety information for Calpain Inhibitor I
Signal word | Warning |
Pictogram(s) |
Exclamation Mark Irritant GHS07 |
GHS Hazard Statements |
H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation |
Precautionary Statement Codes |
P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection. |
Computed Descriptors for Calpain Inhibitor I
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