BROMOSULFALEIN
- CAS NO.:71-67-0
- Empirical Formula: C20H11Br4NaO10S2
- Molecular Weight: 818.02
- MDL number: MFCD00012442
- EINECS: 200-761-0
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-08-04 20:11:12
What is BROMOSULFALEIN?
Chemical properties
white crystalline solid
The Uses of BROMOSULFALEIN
Sulfobromophthalein sodium salt hydrate is a reagent for the quantitative determination of proteins, a dye for liver diagnostics in vitro.
What are the applications of Application
Bromosulfalein is a reagent for the quantitative determination of proteins
Definition
ChEBI: An organic sodium salt that is the disodium salt of bromosulfophthalein.
Purification Methods
Purify it by TLC on silica Gel G (Merck 250a particle size) in two solvent systems (BuOH/AcOH/H2O 30:7.5:12.5 v/v, and BuOH/propionic acid/H2O 30:20:7.5 v/v). When the solvent reaches a height of ~10cm, the plate is removed, dried in air and developed with NH3 vapour giving blue-coloured spots. Also, the dye can be chromatographed on MN Silica Gel with t-BuOH/H2O/n-BuOH (32:10:5 v/v) as eluent and visualised with a dilute KOH (or NaOH if the Na salt is required) spray. The product corresponding to bromosulfalein is scraped off and eluted with H2O, filtered and evaporated to dryness in a vacuum. It was dissolved in H2O and filtered through Sephadex G-25 and evaporated to dryness. [UV and IR identification: Barbier & DeVeerdt J Pharm Sci 57 819 1968, NMR: Kato et al. Chem Pharm Bull Jpn 20 581 1972, McGuire Anal Biochem 83 75 1977, Beilstein 18/9 V 461.]
Properties of BROMOSULFALEIN
solubility | DMF: 5 mg/ml; DMSO: 5 mg/ml; Ethanol: slightly soluble; PBS (pH 7.2): 1 mg/ml |
form | Powder |
color | White to off-white |
Water Solubility | Soluble in water. |
Sensitive | Moisture Sensitive |
Merck | 14,8954 |
Stability: | Stable. Incompatible with strong oxidizing agents. May be air or moisture sensitive. |
EPA Substance Registry System | Benzenesulfonic acid, 3,3'-(4,5,6,7-tetrabromo-3-oxo-1(3H)-isobenzofuranylidene)bis[6-hydroxy-, disodium salt (71-67-0) |
Safety information for BROMOSULFALEIN
Computed Descriptors for BROMOSULFALEIN
Abamectin manufacturer
Pallav Chemicals And Solvents Pvt Ltd
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