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HomeProduct name listBROMOSULFALEIN

BROMOSULFALEIN

  • CAS NO.:71-67-0
  • Empirical Formula: C20H11Br4NaO10S2
  • Molecular Weight: 818.02
  • MDL number: MFCD00012442
  • EINECS: 200-761-0
  • SAFETY DATA SHEET (SDS)
  • Update Date: 2024-08-04 20:11:12
BROMOSULFALEIN Structural

What is BROMOSULFALEIN?

Chemical properties

white crystalline solid

The Uses of BROMOSULFALEIN

Sulfobromophthalein sodium salt hydrate is a reagent for the quantitative determination of proteins, a dye for liver diagnostics in vitro.

What are the applications of Application

Bromosulfalein is a reagent for the quantitative determination of proteins

Definition

ChEBI: An organic sodium salt that is the disodium salt of bromosulfophthalein.

Purification Methods

Purify it by TLC on silica Gel G (Merck 250a particle size) in two solvent systems (BuOH/AcOH/H2O 30:7.5:12.5 v/v, and BuOH/propionic acid/H2O 30:20:7.5 v/v). When the solvent reaches a height of ~10cm, the plate is removed, dried in air and developed with NH3 vapour giving blue-coloured spots. Also, the dye can be chromatographed on MN Silica Gel with t-BuOH/H2O/n-BuOH (32:10:5 v/v) as eluent and visualised with a dilute KOH (or NaOH if the Na salt is required) spray. The product corresponding to bromosulfalein is scraped off and eluted with H2O, filtered and evaporated to dryness in a vacuum. It was dissolved in H2O and filtered through Sephadex G-25 and evaporated to dryness. [UV and IR identification: Barbier & DeVeerdt J Pharm Sci 57 819 1968, NMR: Kato et al. Chem Pharm Bull Jpn 20 581 1972, McGuire Anal Biochem 83 75 1977, Beilstein 18/9 V 461.]

Properties of BROMOSULFALEIN

solubility  DMF: 5 mg/ml; DMSO: 5 mg/ml; Ethanol: slightly soluble; PBS (pH 7.2): 1 mg/ml
form  Powder
color  White to off-white
Water Solubility  Soluble in water.
Sensitive  Moisture Sensitive
Merck  14,8954
Stability: Stable. Incompatible with strong oxidizing agents. May be air or moisture sensitive.
EPA Substance Registry System Benzenesulfonic acid, 3,3'-(4,5,6,7-tetrabromo-3-oxo-1(3H)-isobenzofuranylidene)bis[6-hydroxy-, disodium salt (71-67-0)

Safety information for BROMOSULFALEIN

Computed Descriptors for BROMOSULFALEIN

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