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HomeProduct name listBCIP/NBT

BCIP/NBT

Synonym(s):5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride solution;BCIP/NBT Alkaline Phosphatase Substrate;BCIP/NBT Alkaline Phosphatase Substrate solution;BCIP/NBT Substrate;chromogenic substrate

  • Empirical Formula: C48H34BrClN11O10P
  • Molecular Weight: 1071.18
  • MDL number: MFCD00466954
  • Update Date: 2023-04-23 13:52:06
BCIP/NBT Structural

What is BCIP/NBT?

The Uses of BCIP/NBT

A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures.

The Uses of BCIP/NBT

SIGMAFAST BCIP?/NBT has been used as a substrate

  • for the anti-mouse IgG conjugated alkaline phosphatase in immunoblotting
  • for alkaline phosphatase in the histochemical staining of primary osteoblasts
  • for alkaline phosphatase staining in bone marrow stromal cells

General Description

BM purple is a chromogenic substrate for alkaline phosphatase (AP) designed for precipitating enzyme immunoassays. It develops a permanent, dark purple band or spot at the AP binding site on the membrane or solid support. The stabilized substrate solution may be used directly from the bottle with no mixing or reconstitution. It gives less background staining than when using the BCIP/NBT two-bottle system, without a loss of sensitivity. The reaction product has a dark purple color and is insoluble in water. The dark purple color can be visually seen.

Appearance prior to use: light yellow color;
After color development: dark purple

Properties of BCIP/NBT

Flash point: 67 °C
storage temp.  -20°C
form  tablet

Safety information for BCIP/NBT

Signal word Warning
Pictogram(s)
ghs
Exclamation Mark
Irritant
GHS07
GHS Hazard Statements H315:Skin corrosion/irritation
H319:Serious eye damage/eye irritation
H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes P261:Avoid breathing dust/fume/gas/mist/vapours/spray.
P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

Computed Descriptors for BCIP/NBT

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