5-METHYLCYTOSINE
- CAS NO.：554-01-8
- Empirical Formula: C5H7N3O
- Molecular Weight: 125.13
- MDL number: MFCD00233537
- EINECS: 209-058-3
- SAFETY DATA SHEET (SDS)
- Update Date: 2024-11-19 23:02:33
What is 5-METHYLCYTOSINE?
Chemical properties
Crystals in prisms from water.
The Uses of 5-METHYLCYTOSINE
5-Methylcytosine is used as a marker to protect DNA from being cut by native methylation-sensitive restriction enzymes. It is also employed in the production of 4-amino-5-hydroxy-5-methyl-2-oxo-2,5-dihydro-imidazole-1-carbaldehyde.
Definition
ChEBI: A pyrimidine that is a derivative of cytosine, having a methyl group at the 5-position.
Purification Methods
Crystallise it from water (solubility is 3.4%). The hydrochloride has m 299-301o (sintering at 280o) (from aqueous HCl/Me2CO). [Hitchings et al. J Biol Chem 177 537 1949, Cohn J Am Chem Soc 73 1539 1951, Beilstein 25 II 183, 25 III/IV 3727.]
Properties of 5-METHYLCYTOSINE
| Melting point: | 270°C (rough estimate) |
| Boiling point: | 232.57°C (rough estimate) |
| Density | 1.2602 (rough estimate) |
| refractive index | 1.6510 (estimate) |
| storage temp. | Keep in dark place,Inert atmosphere,Room temperature |
| solubility | Methanol (Sparingly, Heated) |
| pka | 9.05±0.10(Predicted) |
| form | Crystalline |
| color | White to Off-White |
| Water Solubility | Soluble in water. |
| Merck | 14,6050 |
| CAS DataBase Reference | 554-01-8(CAS DataBase Reference) |
Safety information for 5-METHYLCYTOSINE
Computed Descriptors for 5-METHYLCYTOSINE
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