4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE

Synonym(s):4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside;4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-gluco-pyrano-side;4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside;4-Methylumbelliferyl-N-acetyl-β-D-glucosaminide - CAS 37067-30-4 - Calbiochem

CAS NO.：37067-30-4
Empirical Formula: C18H21NO8
Molecular Weight: 379.37
MDL number: MFCD00065481
EINECS: 253-333-0
SAFETY DATA SHEET (SDS)
Update Date: 2023-06-08 09:02:58

4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE Structural

What is 4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE?

Chemical properties

4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE is White Crystalline Solid

The Uses of 4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE

4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE is used for the assay of b-Acetylglucosaminidase. A fluorogenic substrate used for the diagnosis of GM2 gangliosidoses in leukocytes. Genetic defects in human b-hexosaminidase isozy mes (Hex A & Hex B) result in various clinical and biochemical types of GM2 gangliosidosis. They include Tay Sachs disease, Sandhoff disease and other forms. Fluorescence: max. Abs. l = 325nm, max. Em. l = 378nm

The Uses of 4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE

4-Methylumbelliferyl N-acetyl-β-D-glucosaminide has been used as a substrate for assaying

β-N-acetylglucosaminidase (NAGase) from soil samples
chitinase from Fusarium oxysporum using fluorimetric assay
β-N-acetylhexosaminidases from Arabidopsis thaliana roots and leaves samples

General Description

4-Methylumbelliferyl N-acetyl-β-D-glucosaminide (4-MUF-NAG) contains chitin monomer, N-acetyl-D-glucosamine (NAG) and a 4-methylumbelliferone (7-hydroxy-4-methylcoumarin) (4-MUF) moiety.

Biochem/physiol Actions

4-Methylumbelliferyl N-acetyl-β-D-glucosaminide (4-MUF-NAG) is a synthetic fluorogenic substrate for N-acetyl-β-D-glucosaminidase (NAGase). The enzyme functionality is assessed by its ability to hydrolyze the N-acetyl-D-glucosamine (NAG) residue in 4-MUF-NAG. 4-MUF-NAG is also a substrate for Chitinase and N-acetylhexosaminidase.

Properties of 4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE

Melting point:	208-210 °C
Boiling point:	507.24°C (rough estimate)
Density	1.4163 (rough estimate)
refractive index	1.5740 (estimate)
storage temp.	-20°C
solubility	DMF: 20 mg/mL, clear, colorless
form	Crystalline solid
pka	12.87±0.70(Predicted)
color	White to Off-White
λmax	360nm(Phosphate buffer sol.)(lit.)
BRN	1693397
Stability:	Light Sensitive - Protect from Light
CAS DataBase Reference	37067-30-4(CAS DataBase Reference)
EPA Substance Registry System	2H-1-Benzopyran-2-one, 7-[[2-(acetylamino)-2-deoxy-.beta.-D-glucopyranosyl]oxy]-4-methyl- (37067-30-4)

Safety information for 4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE

Signal word	Danger
Pictogram(s)	Flame Flammables GHS02 Corrosion Corrosives GHS05
GHS Hazard Statements	H226:Flammable liquids H314:Skin corrosion/irritation
Precautionary Statement Codes	P210:Keep away from heat/sparks/open flames/hot surfaces. — No smoking. P233:Keep container tightly closed. P240:Ground/bond container and receiving equipment. P241:Use explosion-proof electrical/ventilating/lighting/…/equipment. P242:Use only non-sparking tools. P243:Take precautionary measures against static discharge. P264:Wash hands thoroughly after handling. P264:Wash skin thouroughly after handling. P280:Wear protective gloves/protective clothing/eye protection/face protection. P363:Wash contaminated clothing before reuse. P301+P330+P331:IF SWALLOWED: Rinse mouth. Do NOT induce vomiting. P303+P361+P353:IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower. P370+P378:In case of fire: Use … for extinction. P405:Store locked up. P403+P235:Store in a well-ventilated place. Keep cool.