4-Methylumbelliferyl-beta-D-glucopyranoside
Synonym(s):MUD
- CAS NO.:18997-57-4
- Empirical Formula: C16H18O8
- Molecular Weight: 338.31
- MDL number: MFCD00063694
- EINECS: 242-736-7
- SAFETY DATA SHEET (SDS)
- Update Date: 2023-09-04 15:51:00
What is 4-Methylumbelliferyl-beta-D-glucopyranoside?
Description
4-Methylumbelliferyl-β-D-Glucopyranoside (4-MUG) is a fluorogenic substrate of β-glucosidase and β-glucocerebrosidase (also known as glucosylceramidase). In addition to its use in characterizing novel β-glucosidases, 4-MUG is used in assays to evaluate deficiency in β- glucocerebrosidase activity related to Gaucher disease. Hydrolysis of 4-MUG releases the fluorescent product 4-MU, which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively.
Chemical properties
White Powder
The Uses of 4-Methylumbelliferyl-beta-D-glucopyranoside
A fluorogenic substrate in the assay of ?-glucosidase. Fluorescence: max. Abs. 316nm; max. Em. 372nm; e x 10-3: 15
The Uses of 4-Methylumbelliferyl-beta-D-glucopyranoside
A fluorogenic substrate in the assay of ?-glucosidase.Fluorescence: max. Abs. 316nm; max. Em. 372nm; e x 10-3: 15
The Uses of 4-Methylumbelliferyl-beta-D-glucopyranoside
4-Methylumbelliferyl β-D-glucopyranoside has been used as substrate:
- in glucosylceramidase β enzyme activity assay in lysosome-enriched fractions from primary hippocampal neurons
- in β-glucosidase assay during yeast fermentation
- to assay glucocerebrosidase 1 (GBA1)-related glucosidase activity in macrophage cell line (RAW)
What are the applications of Application
4-Methylumbelliferyl-β-D-glucopyranoside is a fluorogenic substrate for β-glucosidase
Definition
ChEBI: A beta-D-glucoside having a 4-methylumbelliferyl substituent at the anomeric position.
Biochem/physiol Actions
4-Methylumbelliferyl β-D-glucopyranoside is a synthetic enzymatic substrate for glycosidase. It has been used as substrate for the β- glucosidase from enterococci.
Purification Methods
4-Methylumbellifer-7-yl--Dglucopyranoside crystallises as the half hydrate from hot H2O. [Constantzas & Kocourek Collect Czech Chem Commun 24 1099 1959, De Re et al. Ann Chim (Rome) 4 9 2089 1959, Courtin-Duchateau & Veyrière Carbohydr Research 65 23, 29 1978, Beilstein 18 III/IV 5152, 8 IV 433, 18/7 V 616.]
Properties of 4-Methylumbelliferyl-beta-D-glucopyranoside
Melting point: | 258-263 °C (dec.) |
Boiling point: | 394.46°C (rough estimate) |
Density | 1.522 |
refractive index | 1.4430 (estimate) |
storage temp. | Sealed in dry,Store in freezer, under -20°C |
solubility | DMF: 5 mg/ml; DMSO: 50 mg/ml |
pka | 12.73±0.70(Predicted) |
form | Powder |
color | White to off-white |
Water Solubility | Soluble in DMF or water |
λmax | 320nm(Buffer)(lit.) |
BRN | 94672 |
CAS DataBase Reference | 18997-57-4(CAS DataBase Reference) |
Safety information for 4-Methylumbelliferyl-beta-D-glucopyranoside
Signal word | Warning |
Pictogram(s) |
Exclamation Mark Irritant GHS07 |
GHS Hazard Statements |
H302:Acute toxicity,oral H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation |
Precautionary Statement Codes |
P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P280:Wear protective gloves/protective clothing/eye protection/face protection. P301+P312:IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. |
Computed Descriptors for 4-Methylumbelliferyl-beta-D-glucopyranoside
New Products
6-Bromo 2-Iodo Indole Pyridine 2,4,6-Tricarboxaldehyde Fosfomycin EP impurity D Ivermectin EP Impurity B Rimegepant Impurity 3 Tubulysin A Tubulysin B Tubulysin E (2S,4R)-4-amino-2-methyl-5-phenylpentanoic acid hydrochloride Potassium HMDS (1.0 M in THF) Diethyl Aluminium Cyanide (1.0 M in Toluene) Fuel shell (1R,2S)-2-(3,4-Difluorophenyl)cyclopropanamine 2-(1-(Mercaptomethyl) cyclopropyl) acetonitrile 2-[[(3aR,4S,6R,6aS)-6-Aminotetrahydro-2,2-dimethyl-4H-cyclopenta-1,3-dioxol-4-yl]oxy]ethanol ethanedioate 2-[2-[3(S)-3[2-(7-chloro-2-quinolinyl) ethenyl] phenyl-3- hydroxyl propyl] phenyl]-2-propanol Imeglimin Hydrochloride IH 5-Aminophthalazine 3,3,6,6-Tetramethylcyclohexane-1,2-dione 3-Morpholino-1-[4-(2-oxo-1-piperidyl)phenyl]-5,6-dihydropyridin-2(1H)-one Diethyl 2,5-di(furan-2-yl)terephthalate 2-(2,4-Dibutoxyphenyl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane 3,6-Bis(4-iodophenyl)-2,5-dihydropyrrolo[3,4-c]pyrrole-1,4-dione 5-amino-1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-1H-imidazole-4-carbonitrileRelated products of tetrahydrofuran
You may like
-
4-Methylumbelliferyl beta-D-glucopyranoside CAS 18997-57-4View Details
18997-57-4 -
4-Methylumbelliferyl-ß-D- Glucopyranoside extrapure CAS 18997-57-4View Details
18997-57-4 -
4-Methylumbelliferyl β-D-Glucopyranoside CAS 18997-57-4View Details
18997-57-4 -
4-Methylumbelliferyl β-D-glucopyranoside CAS 18997-57-4View Details
18997-57-4 -
Sidchem BT 20View Details
-
1572177-89-9View Details
1572177-89-9 -
6390-09-6View Details
6390-09-6 -
6-Bromo indoleView Details