4-CHLORO-1-NAPHTHOL

Synonym(s):4-chloronaphthalen-1-ol;4-CN

CAS NO.：604-44-4
Empirical Formula: C10H7ClO
Molecular Weight: 178.62
MDL number: MFCD00003974
EINECS: 210-068-5
SAFETY DATA SHEET (SDS)
Update Date: 2025-10-27 16:43:40

What is 4-CHLORO-1-NAPHTHOL?

Chemical properties

Off white to tan crystals

The Uses of 4-CHLORO-1-NAPHTHOL

4-Chloro-1-naphthol has been used to visualize protein bands in western blotting. It has also been used in staining polyvinylidene fluoride (PVDF) membrane by Nakane method.

The Uses of 4-CHLORO-1-NAPHTHOL

Suitable for visualizing protein-peroxidase conjugates in western blotting.

What are the applications of Application

4-Chloro-1-naphthol is a chromogenic peroxidase substrate enzyme-linked detection procedures

Synthesis Reference(s)

Tetrahedron, 38, p. 2347, 1982 DOI: 10.1016/0040-4020(82)87012-9

General Description

4-Chloro-1-naphthol is primarily used for the detection of proteins, it gives a characteristic purple precipitate and the reactions are easily controllable. It can be used as a substitute for benzidine compounds, which are considered to be carcinogenic.

Biochem/physiol Actions

4-Chloro-1-naphthol serves as a substrate for horseradish peroxidase.

Purification Methods

Crystallise the naphthol from EtOH or CHCl3. [Beilstein 6 H 611, 6 II 582, 6 III 2933, 6 IV 4233.]

Properties of 4-CHLORO-1-NAPHTHOL

Melting point:	118-121 °C(lit.)
Boiling point:	254.41°C (rough estimate)
Density	密度
refractive index	1.4575 (estimate)
Flash point:	73 °C
storage temp.	-20°C
solubility	methanol: 50 mg/mL, clear, colorless to very faintly yellow
form	tablets (~32 mg each)
pka	9.11±0.40(Predicted)
color	Off-white to gray to light brown
BRN	509750
CAS DataBase Reference	604-44-4(CAS DataBase Reference)
EPA Substance Registry System	1-Naphthalenol, 4-chloro- (604-44-4)

Safety information for 4-CHLORO-1-NAPHTHOL

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P264:Wash hands thoroughly after handling. P264:Wash skin thouroughly after handling. P280:Wear protective gloves/protective clothing/eye protection/face protection. P304+P340:IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. P405:Store locked up.