periplocin

Name

3-[(3S,5S,10R,13R,14S,17R)-3-[(2R,5R)-6-[[(3R,6S)-4,6-dihydroxy-2-methyloxan-3-yl]oxymethyl]-3,5-dihydroxy-4-methoxyoxan-2-yl]oxy-5,14-dihydroxy-10,13-dimethyl-2,3,4,6,7,8,9,11,12,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-17-yl]-2H-furan-5-one

Synonym

Description

Periplocin is a cardiotonic steroid isolated from Periploca forrestii. Periplocin promotes tumor cell apoptosis and inhibits tumor growth. Periplocin has the potential to facilitate wound healing through the activation of Src/ERK and PI3K/Akt pathways mediated by Na/K-ATPase[1][2].

Related Catalog

Target

Apoptosis[2]

In Vitro

Periplocin (5-20 μM; 48 hours; L929 cells) treatment shows increased proliferation up to 131% at 20 μM[1]. Periplocin (5-20 μM; 30-120 minutes; L929 cells) increases phosphorylation of Src, ERK, PI3K and Akt at active sites in a dosedependent and time-dependent manner.Periplocin (5-20 μM; 48 hours) significantly promotes migration of fibroblast cell[1]. Periplocin (5-20 μM; 48 hours) increases collagen production in L929 fibroblast[1]. Periplocin induces Na/KATPase mediates the activation of Src/ERK and PI3K/Akt pathways[1]. Cell Viability Assay[1] Cell Line: L929 cells Concentration: 5 μM, 10 μM, 20 μM Incubation Time: 48 hours Result: Showed increased proliferation up to 131% at 20 μM. Western Blot Analysis[1] Cell Line: L929 cells Concentration: 5 μM, 10 μM, 20 μM Incubation Time: 30 minutes, 60 minutes, 120 minutes Result: Increased phosphorylation of Src, ERK, PI3K and Akt at active sites in a dosedependent and time-dependent manner.

In Vivo

Periplocin (5-20 mg/kg; intraperitoneal injection; daily; for 14 days; female SCID mice) treatment represses the growth of hepatocellular carcinoma (HCC) in xenograft tumor model in mice[2]. Animal Model: Female SCID mice (6-8 weeks old) injected with Huh-7 cells[2] Dosage: 5 mg/kg, 20 mg/kg Administration: Intraperitoneal injection; daily; for 14 days Result: Repressed the growth of hepatocellular carcinoma (HCC) in xenograft tumor model in mice.

References

[1]. Chen L, et al. Periplocin promotes wound healing through the activation of Src/ERK and PI3K/Akt pathways mediated by Na/K-ATPase. Phytomedicine. 2019 Apr;57:72-83.

[2]. Cheng CF, et al. Antitumor Effect of Periplocin in TRAIL-Resistant Human Hepatocellular Carcinoma Cells through Downregulation of IAPs. Evid Based Complement Alternat Med. 2013;2013:958025.

Density

1.4±0.1 g/cm3

Boiling Point

877.4±65.0 °C at 760 mmHg

Melting Point

205°C

Molecular Formula

C36H56O13

Molecular Weight

682.795

Flash Point

272.8±27.8 °C

Exact Mass

682.356445

PSA

193.83000

LogP

-1.36

Vapour Pressure

0.0±0.6 mmHg at 25°C

Index of Refraction

1.624